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Your Position: Home > Virus > Glycoprotein H & Glycoprotein L (EBV) > GHL-E5283

Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag (MALS verified)

  • Source
    Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer, Twin-Strep&His Tag(GHL-E5283) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Leu 682 (gH) & Asn 23 - Gly 137 (gL) (Accession # P03231 (gH) & P03212 (gL)).
    Predicted N-terminus: Ser 19 & Asn 23
  • Molecular Characterization

    Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag , has a calculated MW of 76.9 kDa (gH) & 14.6 kDa (gL). Subunit gH is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit gL contains no tag but a basic tail at the C-terminus.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Glycoprotein H & Glycoprotein L (EBV) SDS-PAGE

Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer, Twin-Strep&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Glycoprotein H & Glycoprotein L (EBV) MALS images

The purity of Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer, Twin-Strep&His Tag (Cat. No. GHL-E5283) is more than 90% and the molecular weight of this protein is around 100-125kDa verified by SEC-MALS.

Bioactivity-SPR
 Glycoprotein H & Glycoprotein L (EBV) SPR

Human EphA2 Protein, His Tag (Cat. No. EP2-H52H4) immobilized on CM5 Chip can bind Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer, Twin-Strep&His Tag (Cat. No. GHL-E5283) with an affinity constant of 12 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

 Glycoprotein H & Glycoprotein L (EBV) SPR

Human EphA2 Protein, His Tag (Cat. No. EP2-H52H4) immobilized on CM5 Chip can bind Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer, Twin-Strep&His Tag (Cat. No. GHL-E5283) with an affinity constant of 12 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Epstein-Bar Virus (EBV), belonging to gamma herpesvirus, is one of the most common human viruses which widely spread all over the world. EBV is the primary cause of infectious mononucleosis (IM) and is found to be related to several types of cancers. Although gp350 is the most abundant glycoprotein on viruses’ surfaces, the research on using gp350 as vaccine antigen progresses slowly making glycoprotein H and glycoprotein L heterodimer (gH/gL) be considered as a candidate for vaccine development. gH/gL plays an important role in assisting the fusion process with the binding of Ephrin type-A receptor 2 (EphA2).
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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