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Your Position: Home > Virus > Glycoprotein B > GLB-M330

Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1 (MALS verified)

  • Source
    The antibody is recombinantly produced from HEK293 cells.
  • Isotype
    Human IgG1/kappa
  • Specificity
    This antibody has high binding affinity with glycoprotein B of multiple HCMV strains, i.e., strain AD169, strain Merlin, strain Towne.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 51 mM Tris,100 mM Glycine,25 mM Argine,150 mM NaCl,pH7.5 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1 (Cat. No. GLB-M330) SDS-PAGE gel

Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1 (Cat. No. ) MALS images

The purity of Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1 (Cat. No. GLB-M330) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

Bioactivity-ELISA
Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1 (Cat. No. GLB-M330) ELISA bioactivity

Immobilized HCMV (strain AD169) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H4) at 1 μg/mL (100 μL/well) can bind Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1 (Cat. No. GLB-M330) with a linear range of 0.2-16 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1 (Cat. No. GLB-M330) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind HCMV (strain AD169) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H4) with an affinity constant of 3.48 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Human cytomegalovirus is a species of the Cytomegalovirus genus of viruses, which in turn is a member of the viral family known as Herpesviridae or herpesviruses. It is typically abbreviated as HCMV or, commonly but more ambiguously, as CMV. CMV Virus Envelope Glycoportein B (CMV-GB) can be cleaved into glycoprotein GP55. Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. CMV-GB may be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Furthermore, CMV-GB can interact with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Also, CMV-GB participates in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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