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Your Position: Home > Fc Receptor Proteins > Fc gamma RIIB / CD32b > Human Fc gamma RIIB / CD32b Protein, His Tag (SPR & BLI verified)

Human Fc gamma RIIB / CD32b Protein, His Tag (SPR & BLI verified)

  • Synonym
    FCGR2B,CD32b,CD32,FCG2,IGFR2,CDw32
  • Source
    Human CD32b, His Tag (SPR & BLI verified) (CDB-H5228) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994).
    Predicted N-terminus: Ala 46
  • Molecular Characterization
    Online(Ala 46 - Pro 217) P31994

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 20.4 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-H5228) SDS-PAGE gel

Human CD32b, His Tag (SPR & BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Immobilized Human CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-H5228) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 10.1 μM as determined in a SPR assay (Biacore T200) (QC tested).

Human_FcRn_Heterodimer_Protein_SPR

Immobilized Rituximab on CM5 Chip, can bind Human CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-H5228) with an affinity constant of 5.5 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-H5228) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 4.30 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Rituximab on FAB2G Biosensor, can bind Human CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-H5228) with an affinity constant of 5.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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