This is the new website for acrobiosystems.com. We would love to hear what you think of it - please send feedback

Welcome to ACROBiosystems

0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > MABSol® Fc Receptors > Human FcGR2B /CD32b Protein

Human Fc gamma RIIB / CD32b Protein

  • Synonym
    FCGR2B,CD32b,CD32,FCG2,IGFR2,CDw32
  • Source
    Human Fc gamma RIIB, His Tag (CDB-H5228) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994).
    Predicted N-terminus: Ala 46
  • Molecular Characterization
    CD32b(Ala 46 - Pro 217) P31994

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 20.4 kDa. The protein migrates as 23-33 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Fc gamma RIIB, His Tag (Cat. No. CDB-H5228) SDS-PAGE gel

Human Fc gamma RIIB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human Fc gamma RIIB, His TagHuman Fc gamma RIIB, His Tag (Cat. No. CDB-H5228) ELISA bioactivity

Immobilized Anti-Human CTLA4 / CD152, Human IgG1 at 10 μg/mL (100 μL/well) can bind Human CD32b, His Tag (Cat. No. CDB-H5228) with a linear range of 0.156-2.5 μg/mL (QC tested).

  • Background
    Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

Order Details

Price(USD) : $260.00

Inventory : In stock

Price(USD) : $1600.00

Inventory : In stock

Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Related Products

Questions & Comments

This web search service is supported by Google Inc.