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Your Position: Home > All Other Proteins > Carbonic Anhydrase IX > CA9-H5226

Human Carbonic Anhydrase IX / CA9 (38-414) Protein, His Tag

  • Synonym
    CAIX,CA9,CA-IX,G250,MN,P54/58N,pMW1
  • Source
    Human Carbonic Anhydrase IX (38-414), His Tag (CA9-H5226) is expressed from human 293 cells (HEK293). It contains AA Gln 38 - Asp 414 (Accession # NP_001207).
    Predicted N-terminus: Gln 38
  • Molecular Characterization
    Online(Gln 38 - Asp 414) NP_001207

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 41.2 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM MES, 100 mM NaCl, pH6.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Carbonic Anhydrase IX (38-414), His Tag (Cat. No. CA9-H5226) SDS-PAGE gel

Human Carbonic Anhydrase IX (38-414), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

The specific activity is >200 pmol/min/μg, as measured with 4-Nitrophenyl acetate (Routinely tested). 

One unit is defined as the amount of enzyme that hydrolyze 1.0 p mole of 4-Nitrophenyl acetate to 4-Nitrophenol per minute at pH7.5 at Room temperature

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