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Your Position: Home > TME Relevant Pathway > EDIL3 > ED3-H5259

Human EDIL3 Protein, Fc Tag (MALS verified)

  • Synonym
    DEL1,EDIL3
  • Source
    Human EDIL3, Fc Tag (ED3-H5259) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Glu 480 (Accession # O43854-1).
    Predicted N-terminus: Asp 24
  • Molecular Characterization
    Online(Asp 24 - Glu 480) O43854-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 77.8 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human EDIL3, Fc Tag (Cat. No. ED3-H5259) SDS-PAGE gel

Human EDIL3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human EDIL3, Fc Tag (Cat. No. ) MALS images

The purity of Human EDIL3, Fc Tag (Cat. No. ED3-H5259) is more than 85% and the molecular weight of this protein is around 85-115 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human EDIL3, Fc TagHuman EDIL3, Fc Tag (Cat. No. ED3-H5259) ELISA bioactivity

Immobilized Human EDIL3, Fc Tag (Cat. No. ED3-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB3 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT3-H82W9) with a linear range of 0.039-1.25 μg/mL (QC tested).

Human EDIL3, Fc TagHuman EDIL3, Fc Tag (Cat. No. ED3-H5259) ELISA bioactivity

Immobilized Biotinylated Human ITGAV&ITGB3 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT3-H82W9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human EDIL3, Fc Tag (Cat. No. ED3-H5259) with a linear range of 0.3-39 ng/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human EDIL3, Fc Tag (Cat. No. ED3-H5259) on Protein A Biosensor, can bind Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT3-H52E3) with an affinity constant of 533 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    EDIL3,also known as Del1, is a novel extracellular matrix protein encoding three Notch-like epidermal growth factor repeats, an RGD motif, and two discoidin domains. EDIL3 is expressed in an endothelial cell-restricted pattern during early development. ED
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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