Login Register
icon_bulk_orderBulk order Acrobiosystems for English
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Antibody isotype kits > Spike RBD > RAS-T078

Anti-SARS-CoV-2 (B.1.617.2) Antibody IgG3 Titer Serologic Assay Kit (Spike RBD)

For research use only.
Materials Provided
IDComponentsSize
RAS078-C01Pre-coated SARS-CoV-2 (B.1.617.2) Spike RBD Microplate1 plate
RAS078-C02Anti-SARS-CoV-2 Antibody (Control, IgG3)10 μg
RAS078-C03HRP-Mouse anti-Human IgG350 μL
RAS078-C0410xWashing Buffer50 mL
RAS078-C05Dilution Buffer50 mL
RAS078-C06Substrate Solution12 mL
RAS078-C07Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) poses continual threat to human health due to rapid transmission worldwide. Unprecedented vaccination campaigns have now begun with multiple candidates. To support these studies, ACRO has developed Anti-SARS-CoV-2 subtype antibody Serologic Assay kit including:IgG1, IgG2 IgG3, IgG4.
  • Application
    The kit is developed for qualitative detection or titer measurement of Anti-SARS-CoV-2 (B.1.617.2) antibody IgG3 (Spike RBD) in human serum.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit is used to measure the levels of Anti-SARS-CoV-2 antibody IgG1 by employing an indirect ELISA format. The microplate in the kit has been pre-coated with SARS-CoV-2 Spike RBD. First add the diluted samples to the plate and wash the wells after incubation. Afterwards add HRP-Mouse anti-Human IgG3 to the plate and wash the wells after incubation. Lastly the substrate is loaded into the wells and color develops in proportion to the amount of antibody. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm and 630 nm. The OD Value reflects the amount of antibody bound.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Mouse-Anti-Human IgG3 to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 (B.1.617.2) Antibody IgG3 Titer Serologic Assay Kit (Spike RBD)Anti-SARS-CoV-2 (B.1.617.2) Antibody IgG3 Titer Serologic Assay Kit (Spike RBD) (Cat. No. RAS-T078) ELISA bioactivity

Detection of Anti-SARS-CoV-2 Antibody, Human IgG3 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD (B.1.617.2) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG3 in 1:20 human serum. Detection was performed using HRP-Conjugated Anti-human IgG3 antibody with sensitivity of 2.4 ng/mL (QC tested).

Comments (0)


ETD of in-stock products: 2 business days

Price(USD) : $650.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America: +1 800-810-0816 (Toll Free)
Asia & Pacific: +86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message