This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 63.5 kDa. The protein migrates as 70-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human SIRP alpha, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human SIRP alpha, Fc Tag (HPLC-verified) (Cat. No. SIA-H5251) was greater than 90% as determined by SEC-HPLC.
FACS assay shows that recombinant Human SIRP alpha, Fc Tag (HPLC-verified) (Cat. No. SIA-H5251) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 1 μg/ml (Routinely tested).
Authors: Yu XY, et al.
Journal: Biochimie 2018
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