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Your Position: Home > Non SARS-Cov-2 Kits > IFN-gamma > CRS-A001

Human Interferon-γ(IFN-γ) ELISA Kit

For research use only.
Materials Provided
CRS001-C01Pre-coated Anti-IFN-γ Antibody Microplate1 plate
CRS001-C02Human IFN-γ Standard30 μg
CRS001-C03Biotin-Anti-IFN-γ Antibody200 μL
CRS001-C04Streptavidin-HRP100 μL
CRS001-C0510xWashing Buffer50 mL
CRS001-C062xDilution Buffer50 mL
CRS001-C07Substrate Solution12 mL
CRS001-C08Stop Solution7 mL
  • Background
    Cytokine storm syndromes is an activation cascade of pro-inflammatory cytokines,such as TNF-α,IL-1,IL-6,IL-12,IFN-α,IFN-β,IFN-γ,MCP-1 and IL-8,due to unregulated host immune response system to different stimuli such as infections, malignancy, rheumatoid disorders, drugs, and so on. Interferon-γ (IFN-γ), one of the four subclasses ofinterferons, and is a multipotent protein, acting on many cell types by inducing or inhibiting many cellular functions through direct effects on gene expression.
  • Application
    The kit is developed for quantitative detection of IFN-γ in human serum and cell culture supernates.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human IFN-γ. The kit consists of Pre-coated Anti-IFN-γ Antibody Microplate and Human IFN-γ Standard and Biotin-Anti-IFN-γ Antibody and Streptavidin-HRP and buffers.

    Your experiment will include 6 simple steps:

    a) All reagents were returned to room temperature(20℃-25℃) before use.

    b) Add your sample to the plate, take the Human IFN-γ as standard sample. The samples and standard sample are diluted by Dilution Buffer.

    c) Wash the plate and add a diluted Biotin-Anti-IFN-γ Antibody to the plate. The diluted by Dilution Buffer.

    d) Wash the plate and add a diluted Streptavidin-HRP to the plate. The diluted by Dilution Buffer.

    e) Wash the plate and add TMB.

    f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

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ETD of in-stock products: 2 business days

Price(USD) : $280.00

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