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Your Position: Home > All Other Proteins > NY-ESO-1 > HL1-H82E4

Biotinylated Human HLA-A*02:01 NY-ESO-1 (SLLMWITQV) Complex Protein (Monomer, MALS verified)

Based on the high binding affinity between biotin-streptavidin systems, our best seller SP-11 Streptavidin Coated Plates are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    NY-ESO-1
  • Source
    Biotinylated Human HLA-A*02:01 NY-ESO-1 (SLLMWITQV) Complex Protein (HL1-H82E4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*0201) & SLLMWITQV & Ile 21 - Met 119 (B2M) (Accession # AAA59606.1(HLA-A*0201) & CAA05908.1(NY-ESO-1) & NP_004039.1(B2M)).
    Predicted N-terminus: Gly 25 & Ser
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 36.3 kDa and 13.8 kDa. The protein migrates as 42-45 kDa and 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human HLA-A*02:01 NY-ESO-1 (SLLMWITQV) Complex Protein (Cat. No. HL1-H82E4) SDS-PAGE gel

Biotinylated Human HLA-A*02:01 NY-ESO-1 (SLLMWITQV) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human HLA-A*02:01 NY-ESO-1 (SLLMWITQV) Complex Protein (Cat. No. ) MALS images

The purity of Biotinylated Human HLA-A*02:01 NY-ESO-1 (SLLMWITQV) Complex Protein (Cat. No. HL1-H82E4) is more than 90% and the molecular weight of this protein is around 44-60 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human HLA-A*02:01 NY-ESO-1 (SLLMWITQV) Complex ProteinBiotinylated Human HLA-A*02:01 NY-ESO-1 (SLLMWITQV) Complex Protein (Cat. No. HL1-H82E4) ELISA bioactivity

Immobilized Biotinylated Human HLA-A*02:01 NY-ESO-1 (SLLMWITQV) Complex Protein (Cat. No. HL1-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-NY-ESO-1 Antibody, Human IgG1 with a linear range of 0.2-16 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-NY-ESO-1 antibody captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Biotinylated Human HLA-A*02:01 NY-ESO-1 (SLLMWITQV) Complex Protein (Monomer) (Cat. No. HL1-H82E4) with an affinity constant of 8.36 nM as determined in a SPR assay (Biacore 8K) (QC tested).

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