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Your Position: Home > IgG kits > Spike S1 > RAS-T084

Mouse Anti-SARS-CoV-2 (B.1.351) Antibody IgG Titer Serologic Assay Kit (Spike S1)

For research use only.
IDComponentsSize
RAS084-C01Pre-coated SARS-CoV-2 Spike S1 (B.1.351) Microplate1 plate
RAS084-C02Positive Control100 μL
RAS084-C03Negative Control 100 μL
RAS084-C04HRP-Goat anti-Mouse IgG10 μg
RAS084-C0510xWashing Buffer50 mL
RAS084-C06Dilution Buffer50 mL
RAS084-C07Substrate Solution12 mL
RAS084-C08Stop Solution7 mL
  • Background
    Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide.As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans.To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure IgG antibodies against the mutants is in urgent need.
  • Application
    The kit is developed for qualitative detection or titer measurement of Anti-SARS-CoV-2 (B.1.351) antibody IgG (Spike S1) in mouse serum.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Mouse serum by SARS-CoV-2 Spike S1. The Kit consists of Pre-coated with SARS-CoV-2 Spike S1 Microplate, an Positive Control, an Negative Control, an HRP-Anti-Goat anti mouse IgG secondary antibody.

    Your experiment will include 4 simple steps:

    a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.

    b) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

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Price(USD) : $650.00

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