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Your Position: Home > Immune Checkpoint Proteins > CD30 > Human CD30 /TNFRSF8 Protein, With C-Fc Tag

Human CD30 / TNFRSF8 Protein, Fc Tag

  • Synonym
    TNFRSF8,CD30,D1S166E,Ki-1
  • Source
    Human CD30, Fc Tag (CD0-H5250) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Lys 379 (Accession # NP_001234.2).
    Predicted N-terminus: Phe 19
  • Molecular Characterization
    Online(Phe 19 - Lys 379) NP_001234.2

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 64.6 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD30, Fc Tag (Cat. No. CD0-H5250) SDS-PAGE gel

Human CD30, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human CD30, Fc TagHuman CD30, Fc Tag (Cat. No. CD0-H5250) ELISA bioactivity

Immobilized Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) at 2 μg/mL (100 μL/well) can bind Human CD30, Fc Tag (Cat. No. CD0-H5250) with a linear range of 0.5-31 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human CD30, Fc Tag (Cat. No. CD0-H5250) on Protein A Biosensor, can bind Human CD30 Ligand, His Tag, low endotoxin (Cat. No. CDL-H524b) with an affinity constant of 82.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Price(USD) : $260.00

Price(USD) : $1500.00

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  • Number of Launched Drugs:1 Details
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  • Latest Research Phase:Approved

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