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Your Position: Home > CAR-T > NY-ESO-1 > HL1-H52E8

Human HLA-A*02:01 NY-ESO-1 (SLLMWITQC) Tetramer Protein (MALS verified)

  • Synonym
    NY-ESO-1
  • Source
    Human HLA-A*02:01 NY-ESO-1 (SLLMWITQC) Tetramer Protein (HL1-H52E8) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*0201) & SLLMWITQC & Ile 21 - Met 119 (B2M) (Accession # AAA59606.1(HLA-A*0201) & CAA05908.1(NY-ESO-1) & NP_004039.1(B2M)).
    Predicted N-terminus: Gly 25 & Ser
  • Molecular Characterization

    Human HLA-A*02:01 NY-ESO-1 (SLLMWITQC) Tetramer Protein is assembled by biotinylated monomer and streptavidin. The biotinylated monomer is assembled by co-expression of NY-ESO-1 covalently linked with B2M and HLA. The biotinylated Human NY-ESO-1 (HLA-A*02:01) Complex monomer carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The calculated MW of HLA, B2M and streptavidin are 36.3 kDa, 13.8 kDa and 13.3 kDa, respectively. The protein migrates as 40-43 kDa and 15 kDa and 14 kDa respectively under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human HLA-A*02:01 NY-ESO-1 (SLLMWITQC) Tetramer Protein (Cat. No. HL1-H52E8) SDS-PAGE gel

Human HLA-A*02:01 NY-ESO-1 (SLLMWITQC) Tetramer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human HLA-A*02:01 NY-ESO-1 (SLLMWITQC) Tetramer Protein (Cat. No. ) MALS images

The purity of Human HLA-A*02:01 NY-ESO-1 (SLLMWITQC) Tetramer Protein (Cat. No. HL1-H52E8) is more than 90% and the molecular weight of this protein is around 234-286 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-NY-ESO-1 antibody captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HLA-A0201 NY-ESO-1 (SLLMWITQC) Tetramer Protein (Cat. No. HL1-H52E8) with an affinity constant of 1.35 nM as determined in a SPR assay (Biacore 8K) (QC tested).

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