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| Assay Type | FACS |
| Analyte |
Wild-type Alpha B.1.1.7 Beta B.1.351 Gamma P.1 Delta B.1.617.2 Omicron B.1.1.529 |
| Format | 96T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity |
38.78 ng/mL 42.79 ng/mL 35.7 ng/mL 39.72 ng/mL 35.74 ng/mL 54.74 ng/mL |
| Standard Curve Range | 130~16640 ng/mL |
| Assay Time | 1 hr 34 min |
| Suitable Sample Type | human serum, human plasma |
| Sample volume | 5 μL |
| ID | Components | Size |
| FCM04-C01 | SARS-CoV-2 Antibody Calibrator | 20 μg |
| FCM04-C02 | RBD 6-plex Beads | 200 μL |
| FCM04-C03 | Assay Buffer | 55 mL |
| FCM04-C04 | 5 × Wash Buffer | 10 mL |
| FCM04-C05 | Biotinylated Human ACE2 | 40 μg |
| FCM04-C06 | 5 × PBS Buffer | 10 mL |
| FCM04-C07 | APC Beads | 500 μL |
| FCM04-C08 | PE Beads | 500 μL |
| FCM04-C09 | 96-Well V-bottom Plate | 1 plate |
| FCM04-C10 | 96-Well Sealing Film | 2 pieces |
| FCM04-C11 | SA-PE | 200 μL |
The kit is developed for qualitative detection of Anti-SARS-CoV-2 Variants (Alpha, Beta, Gamma, Delta, Omicron) neutralizing antibody in human serum.
It is for research use only.
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
A representative datapoint showing single bead population in P1 gate (left), SARS-CoV-2 variant immobilized beads classified by APC channel (middle, P2 ~ P7 gate), and measured fluorescence signal for each population in PE channel (right). Data was generated on BD FACSLyricTM Flow Cytometer, equipped with blue laser (488 nm) and red laser (640 nm).
A 4-parameter logistic model fitting curve was plotted on SARS-CoV-2 Variants Neutralizing Antibody 6-plex Panel. Immobilized SARS-CoV-2 variant Spike proteins bind with biotinylated human ACE2, to be competed out by a serial dilution of potent neutralizing antibody calibrator, ranging from 65 ng/ml to 16640 ng/ml. EC50 values indicate the concentration at which 50% of calibrator were bound. (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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