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Your Position: Home > Kits > Spike RBD > FCM-N04R

SARS-CoV-2 Variants Neutralizing Antibody 6-plex Panel (Flow Cytometry Multiplex Bead Assay)

For research use only.

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Product Details
Assay Type FACS
Analyte Wild-type
Alpha B.1.1.7
Beta B.1.351
Gamma P.1
Delta B.1.617.2
Omicron B.1.1.529
Format 96T
Reactivity Human
Regulatory Status RUO
Sensitivity 38.78 ng/mL
42.79 ng/mL
35.7 ng/mL
39.72 ng/mL
35.74 ng/mL
54.74 ng/mL
Standard Curve Range 130~16640 ng/mL
Assay Time 1 hr 34 min
Suitable Sample Type human serum, human plasma
Sample volume 5 μL
Materials Provided
ID Components Size
FCM04-C01 SARS-CoV-2 Antibody Calibrator 20 μg
FCM04-C02 RBD 6-plex Beads 200 μL
FCM04-C03 Assay Buffer 55 mL
FCM04-C04 5 × Wash Buffer 10 mL
FCM04-C05 Biotinylated Human ACE2 40 μg
FCM04-C06 5 × PBS Buffer 10 mL
FCM04-C07 APC Beads 500 μL
FCM04-C08 PE Beads 500 μL
FCM04-C09 96-Well V-bottom Plate 1 plate
FCM04-C10 96-Well Sealing Film 2 pieces
FCM04-C11 SA-PE 200 μL
  • Background
    Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide.As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans.To facilitate the mutant-related research, drug trials and vaccine development, a multiplex serological test for assessing antibody responses to mutant strains is in urgent need.
  • Application

    The kit is developed for qualitative detection of Anti-SARS-CoV-2 Variants (Alpha, Beta, Gamma, Delta, Omicron) neutralizing antibody in human serum.

    It is for research use only.

  • Storage

    1. Unopened kit should be stored at 2℃-8℃ upon receiving.
    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    SARS-CoV-2 Variants Neutralizing Antibody 6-plex Panel is a multiplex bead-based competitive immunofluorescence assay, utilizing flow cytometry for data acquisition and classification. Six SARS-CoV-2 Spike protein (WT / Alpha / Beta / Gamma / Delta / Omicron ) were conjugated to 6 fluorochrome-encoded microspheres respectively. Anti-SARS-CoV-2 neutralizing antibody in blood specimens competes with ACE2, for binding to Spike protein encapsulated 6-plex beads. Streptavidin conjugated R-phycoerythrin (SA-PE) was employed to detect biotinylated ACE2 bound to the beads. The intensity of PE fluorescence was assessed by flow cytometry at wavelength of 575 nm approximately, in inversely proportion to the Anti-SARS-CoV-2 neutralizing antibody in blood specimens. The intensity of APC fluorescence was applied to classify bead populations, at wavelength of 670 nm approximately.
Bioactivity-FACS Please refer to Ds document for the assay protocol.

A representative datapoint showing single bead population in P1 gate (left), SARS-CoV-2 variant immobilized beads classified by APC channel (middle, P2 ~ P7 gate), and measured fluorescence signal for each population in PE channel (right). Data was generated on BD FACSLyricTM Flow Cytometer, equipped with blue laser (488 nm) and red laser (640 nm).


A 4-parameter logistic model fitting curve was plotted on SARS-CoV-2 Variants Neutralizing Antibody 6-plex Panel. Immobilized SARS-CoV-2 variant Spike proteins bind with biotinylated human ACE2, to be competed out by a serial dilution of potent neutralizing antibody calibrator, ranging from 65 ng/ml to 16640 ng/ml. EC50 values indicate the concentration at which 50% of calibrator were bound. (QC tested).

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Price(USD) : $1094.00

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