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Your Position: Home > IgG kits > Spike RBD > FCM-B01R

SARS-CoV-2 Variants Total IgG 6-plex Panel (Flow Cytometry Multiplex Bead Assay)

For research use only.
Product details
Assay TypeFACS
Alpha B.1.1.7
Beta B.1.351
Gamma P.1
Delta B.1.617.2
Omicron BA.1
Regulatory StatusRUO
Sensitivity16.56 ng/mL
18.33 ng/mL
14.64 ng/mL
16.23 ng/mL
15.96 ng/mL
12.81 ng/m
Standard Curve Range32.5~1040 ng/mL
Assay Time1 hr 50 min
Suitable Sample Typehuman serum, human plasma
Sample volume3 μL
Materials provided
FCM01-C02RBD 6-plex Beads200μL
FCM01-C03Assay Buffer40mL×2
FCM01-C045 × Wash Buffer10mL
FCM01-C05Detection Antibody200μL
FCM01-C065 × PBS Buffer10mL
FCM01-C07APC Beads500μL
FCM01-C08PE Beads500μL
FCM01-C0996-Well V-bottom Plate1plate
FCM01-C1096-Well Sealing Film2pieces
  • Background
    Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide.As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans.To facilitate the mutant-related research, drug trials and vaccine development, a multiplex serological test for assessing antibody responses to mutant strains is in urgent need.
  • Application
    The kit is developed for qualitative detection of Anti-SARS-CoV-2 Variants (Alpha, Beta, Gamma, Delta, Omicron) total IgG antibody in human serum.

    It is for research use only.

  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    SARS-CoV-2 Variants Total IgG 6-plex Panel is a multiplex bead-based indirect immunofluorescence assay, utilizing flow cytometry for data acquisition and classification. Six SARS-CoV-2 Spike RBD protein fragments (WT / Alpha / Beta / Gamma / Delta / Omicron) were conjugated to 6 fluorochrome-encoded microspheres respectively. The Anti-SARS-CoV-2 IgG Antibody in blood specimens was bound to the RBD encapsulated 6-plex beads, and then detected by PE-conjugated anti-human IgG detection antibody, forming bead-analyte-detection antibody sandwiches. The intensity of PE fluorescence was assessed by flow cytometry at wavelength of 575 nm approximately, in proportion to the Anti-SARS-CoV-2 IgG titer in blood specimens. The intensity of APC fluorescence was applied to classify bead populations, at wavelength of 670 nm approximately.
*Optional: If there is vacuum manifold for 96-Well plates,  96-Well Filter Plate (ACROBiosystems, cat#FCM-B01M) is prepared.

A representative datapoint showing single bead population in P1 gate (left), SARS-CoV-2 variant immobilized beads classified by APC channel (middle, P2 ~ P7 gate), and measured fluorescence signal for each population in PE channel (right). Data was generated on BD FACSLyricTM Flow Cytometer, equipped with blue laser (488 nm) and red laser (640 nm).


A 4-parameter logistic model fitting curve was plotted on SARS-CoV-2 Variants total IgG 6-plex Panel. Immobilized SARS-CoV-2 variant RBD proteins bind with a serial dilution of potent and broad antibody calibrator, ranging from 16.5 ng/ml to 4160 ng/ml (QC tested). EC50 values indicate the concentration at which 50% of calibrator were bound.

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Price(USD) : $1094.00

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