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Your Position: Home > IgG kits > Spike RBD > FCM-B01R

SARS-CoV-2 Variants Total IgG 6-plex Panel (Flow Cytometry Multiplex Bead Assay)

For research use only.
  • Background
    Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide.As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans.To facilitate the mutant-related research, drug trials and vaccine development, a multiplex serological test for assessing antibody responses to mutant strains is in urgent need.
  • Application
    The kit is developed for qualitative detection of Anti-SARS-CoV-2 Variants (Alpha, Beta, Gamma, Delta, Omicron) total IgG antibody in human serum.
    It is for research use only.
  • Storage

    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles

    NA

*Optional: If there is vacuum manifold for 96-Well plates,  96-Well Filter Plate (ACROBiosystems, cat#FCM-B01M) is prepared.
Bioactivity-FACS
FACS

A representative datapoint showing single bead population in P1 gate (left), SARS-CoV-2 variant immobilized beads classified by APC channel (middle, P2 ~ P7 gate), and measured fluorescence signal for each population in PE channel  (right). Data was generated on BD FACSLyricTM Flow Cytometer, equipped with blue laser (488 nm) and red laser (640 nm).

FACS assay shows that recombinant Biotinylated FACS

A representative calibration curve generated by fitting the signals from the calibrators to a 4-parameter logistic model (4-PL). The MFI signal is proportional to the concentration of SARS-CoV-2 variant specific-IgG, in format of multiplex bead-based flow cytometry immunoassay.

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