This protein carries a polyhistidine tag at the C-terminus, followed by a twin strep tag.
The protein has a calculated MW of 141.2 kDa. The protein migrates as 170-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Bat coronavirus Spike Trimer, His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Bat coronavirus Spike Trimer, His Tag&Twin-Strep Tag (Cat. No. SPN-B5283) is more than 90% and the molecular weight of this protein is around 545-610 kDa verified by SEC-MALS.
Immobilized Bat coronavirus Spike Trimer, His Tag&Twin-Strep Tag (Cat. No. SPN-B5283) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-4 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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