Login Register
icon_bulk_orderBulk order Acrobiosystems for English
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > TME Relevant Pathway > Laminin 221 E8 > LA2-H5269

Human Laminin 221 E8 Protein, Fc Tag (MALS verified)

  • Synonym
    Laminin 221 E8
  • Source
    Human Laminin 221 E8, Fc Tag (LA2-H5269) is expressed from human 293 cells (HEK293). It contains AA Leu 1900 - Ala 2722 (Laminin alpha2E8) & Leu 1573 - Gln 1798 (Laminin beta2E8) & Asn1364 - Pro1609 (Laminin gamma1E8) (Accession # P24043-1(Laminin alpha2E8) & P55268-1(Laminin beta2E8) & P11047-1(Laminin gamma1E8)).
    Predicted N-terminus: Leu 1900 (Laminin alpha2E8) & Pro (Laminin beta2E8) & Pro (Laminin gamma1E8)
  • Molecular Characterization

    Human Laminin 221 E8, Fc Tag, produced by co-expression of Laminin alpha2E8, Laminin beta2E8 and Laminin gamma1E8, has a calculated MW of 94.3 kDa, 51.3 kDa and 53.7 kDa. The non-reducing (NR) protein migrates as 118-130 kDa due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Laminin 221 E8, Fc Tag (Cat. No. LA2-H5269) SDS-PAGE gel

Human Laminin 221 E8, Fc Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human Laminin 221 E8, Fc Tag (Cat. No. ) MALS images

The purity of Human Laminin 221 E8, Fc Tag (Cat. No. LA2-H5269) is more than 85% and the molecular weight of this protein is around 265-295 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human Laminin 221 E8, Fc TagHuman Laminin 221 E8, Fc Tag (Cat. No. LA2-H5269) ELISA bioactivity

Immobilized Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) at 5 μg/mL (100 μL/well) can bind Human Laminin 221 E8, Fc Tag (Cat. No. LA2-H5269) with a linear range of 0.2-20 ng/mL (QC tested).

Human Laminin 221 E8, Fc TagHuman Laminin 221 E8, Fc Tag (Cat. No. LA2-H5269) ELISA bioactivity

Immobilized Human Laminin 221 E8, Fc Tag (Cat. No. LA2-H5269) at 5 μg/mL (100 μL/well) can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) with a linear range of 0.02-0.313 μg/mL (Routinely tested).

  • Background
    Laminin-221 (LM221) takes multiple roles as a scaffold to store cells, growth factors and cytokines and is the most likely expressed cardiac laminin that maintains physiological activities of myocytes. Laminin 221-E8 consists of the C-terminal end of the alpha 2, beta 2, and gamma 1 chains. Previous study has confirmed that the laminin-221 heterotrimer (α2β2γ1) could bind integrin α7X1 and α7X2. Latest research indicated that the administration of LM221 enhanced accumulation of smooth muscle actin (SMA) and isolectin B4 (ILB4) double-positive cells to the damaged area and contributed to the improvement of cardiac function.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)


ETD of in-stock products: 2 business days

Price(USD) : $400.00

Price(USD) : $2600.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America: +1 800-810-0816 (Toll Free)
Asia & Pacific: +86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message