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Your Position: Home > Non SARS-Cov-2 Kits > IFN-gamma > CEA-C006

ClinMax™ Human IFN-γ ELISA Kit

For research use only.
Product details
Assay Type Sandwich-ELISA
Analyte IFN-γ
Format 96T
Reactivity Human
Regulatory Status RUO
Sensitivity <4.0 pg/mL
Standard Curve Range 7.18 pg/mL-1000 pg/mL
Assay Time 2 hr
Suitable Sample Type For the quantitative determination of human IFN-γ in Plasma and Serum.
Sample volume 50 μL
Materials provided
IDComponentsSize
CEA006-C01Pre-coated Anti-IFN-γ Antibody Microplate1 plate
CEA006-C02IFN-γ Calibrator15 μg×2
CEA006-C03Biotin-Anti-IFN-γ Antibody Concentrated Solution100 μL
CEA006-C04Biotin Antibody Dilution Buffer8 mL
CEA006-C05IFN-γ Streptavidin-HRP Concentrated Solution0.5 mL
CEA006-C06Streptavidin-HRP Dilution Buffer15 mL
CEA006-C0720× Washing Buffer50 mL
CEA006-C08Sample Dilution Buffer15 mL×2
CEA006-C09Substrate Solution12 mL
CEA006-C10Stop Solution6 mL
  • Background
    ClinMax™ Human Interferon Gamma (IFN-γ) Quantitative ELISA Kit is a one-step quick immunoassay designed to quantitate human IFN-γ that is present in complex biological matrices, such as human serum, plasma, buffered solution. This assay kit is a standard sandwich-ELISA format.

    ClinMax product are manufactured in a dedicated GMP facility and are compliant with our ISO 13485 quality management system.

    The verification scheme was designed in accordance with the 9012 "Guidelines for the Verification of Quantitative Analysis Methods of Biological Samples" in the Chinese Pharmacopoeia 2020 Edition and the ICH M10 Guideline. Sensitivity, specificity, precision, recovery, and linearity were verified for multi-batch products and multiple concentration samples. See manual for more information on validation in DS.

  • Application
    Results are obtained by Log-Log Linear regression equation are used to draw the standard curve and calculate the sample concentration. The verification results indicate that this kit can be used for the quantitative determination of natural and recombinant human IFN-γ concentrations. It has been calibrated against a highly purified human IFN-γ and is evaluated with standard from NIBSC/WHO. Reference Reagent INTERFERON GAMMA (Human, rDNA derived) NIBSC code: 87/586.

    It is for research use only.

  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
     IFN-gamma Assay Principles
Typical Data
 IFN-gamma ELISA

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CEA-C006 is less than 4.0 pg/mL.

Validation
Dilution Linearity

Three human serum samples with high concentrations of IFN-γ were diluted 1:2, 1:4, 1:8, 1:16, 1:32, 1:64 with Dilution Buffer to produce samples with values within the dynamic range and then assayed. On average, 99.58% of IFN-γ was detected from serum samples.

 IFN-gamma DILUTION LINEARITY
Intra-Assay Statistics

Ten replicates of each of five samples containing different IFN-γ concentrations were tested in one assay, Intra-Assay Precision CV<10%.

 IFN-gamma INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Five samples containing different concentrations of IFN-γ were tested in three independent assays, Inter-Assay Precision CV<15%.

 IFN-gamma INTER-ASSAY STATISTICS
Recovery

Recombinant IFN-γ(8000,6000,4000pg/mL)was spiked into 5 human serum samples, and then analyzed. On average, 98.28% of IFN-γ was recovered from serum samples.

 IFN-gamma RECOVERY
Interference effect

Bilirubin (simulated jaundice) concentration should be less than 20mg/dL, hemoglobin (simulated hemolysis) concentration should not be higher than 3500mg/dL, triglyceride (simulated lipemia) concentration may not be higher than 2.0g/L,EDTA concentration should be less than 4mg/mL, and Sodium citrate concentration may not be higher than 40mg/mL,it does not affect the detection result.

 IFN-gamma INTERFERENCE EFFECT
  • Background: IFN-gamma
    Interferon-γ, IFN-γ, Interferon Gamma
    Interferon-γ is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
    IFN-γ is capable of orchestrating numerous protective functions to heighten immune responses in infections and cancers. It can exhibit its immunomodulatory effects by enhancing antigen processing and presentation, increasing leukocyte trafficking, inducing an anti-viral state, boosting the anti-microbial functions and affecting cellular proliferation and apoptosis.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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