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Your Position: Home > GMP > IL-3 > IL3-H5218

Human IL-3 Protein, premium grade

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ACROBiosystems has established the GMP grade facility and strict quality management system to support the clinical study of immune cell therapy. If you have any customized inquiries of GMP products, please click here to leave your message.
  • Synonym
    IL3,MCGF,MGC79398,MGC79399,MULTI-CSF,Interleukin-3
  • Source
    Human IL-3, premium grade(IL3-H5218) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Phe 152 (Accession # P08700-1 ).
    Predicted N-terminus: Ala 20
    Human IL-3, premium grade (IL3-H5218), designed for preclinical stage, has the same activity and performance with GMP Human IL-3, which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.
  • Molecular Characterization
    IL-3 Structure

    This protein carries no "tag"

    The protein has a calculated MW of 15.1 kDa. The protein migrates as 18-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Mycoplasma
    Negative.
  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-3 SDS-PAGE

Human IL-3, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
IL-3 MALS images

The purity of Human IL-3, premium grade (Cat. No. IL3-H5218) is more than 95% and the molecular weight of this protein is around 28-36 kDa verified by SEC-MALS.

Bioactivity-BLI
 IL-3 BLI

Loaded Human IL-3 R alpha, Fc Tag (Cat. No. ILA-H5252) on Protein A Biosensor, can bind Human IL-3, premium grade (Cat. No. IL3-H5218) with an affinity constant of 0.47 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

  • Background
    Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The human IL-3 reported to be a monomer, as it is known, contains 133 amino acids residues which is a single non-glycosylated polypeptide. Specifically, human and murine IL-3 share low homology and it does not show activity on murine cells.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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