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Your Position: Home > Antibody isotype kits > Spike RBD > RAS-T101

Anti-SARS-CoV-2 (BA.1) Antibody IgA2 Titer Serologic Assay Kit (Spike RBD)

For research use only.
Materials Provided
IDComponentsSize
RAS101-C01Pre-coated SARS-CoV-2 Spike RBD(BA.1) Microplate1 plate
RAS101-C02Anti-SARS-CoV-2 Antibody (Control, IgA2)100 μL
RAS101-C03HRP-Anti-Human IgA250 μL
RAS101-C0410xWashing Buffer50 mL
RAS101-C05Dilution Buffer50 mL
RAS101-C06Substrate Solution12 mL
RAS101-C07Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective Assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
  • Application
    This kit is developed for titer detection of Anti-SARS-CoV-2 (BA.1) Antibody IgA2 (Spike RBD) in human serum.
    It is for research use only.
  • Storage

    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit is used to measure the levels of Anti-SARS-CoV-2 antibody IgA2 by employing an indirect ELISA format. The microplate in the kit has been pre-coated with SARS-CoV-2 Spike RBD(BA.1). First add the diluted samples to the plate and wash the wells after incubation. Afterwards add HRP-Anti-Human IgA2 to the plate and wash the wells after incubation. Lastly the substrate is loaded into the wells and color develops in proportion to the amount of antibody. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm. The OD Value reflects the amount of antibody bound.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Anti-Human IgA1 to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 (BA.1) Antibody IgA2 Titer Serologic Assay Kit (Spike RBD)Anti-SARS-CoV-2 (BA.1) Antibody IgA2 Titer Serologic Assay Kit (Spike RBD) (Cat. No. RAS-T101) ELISA bioactivity

Detection of Anti-SARS-CoV-2 Antibody, Human IgA2 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD(BA.1) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgA2 in 1:100 human serum. Detection was performed using HRP-Conjugated Anti-human IgA2 antibody with sensitivity of 390 ng/mL (QC tested).

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Price(USD) : $650.00

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