|RAS101-C01||Pre-coated SARS-CoV-2 Spike RBD(BA.1) Microplate||1 plate|
|RAS101-C02||Anti-SARS-CoV-2 Antibody (Control, IgA2)||100 μL|
|RAS101-C03||HRP-Anti-Human IgA2||50 μL|
|RAS101-C04||10xWashing Buffer||50 mL|
|RAS101-C05||Dilution Buffer||50 mL|
|RAS101-C06||Substrate Solution||12 mL|
|RAS101-C07||Stop Solution||7 mL|
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Anti-Human IgA1 to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Anti-SARS-CoV-2 Antibody, Human IgA2 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD(BA.1) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgA2 in 1:100 human serum. Detection was performed using HRP-Conjugated Anti-human IgA2 antibody with sensitivity of 390 ng/mL (QC tested).
Price(USD) : $650.00
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