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Your Position: Home > Non SARS-Cov-2 Kits > IL-10 > CEA-C005

ClinMax™ Human IL-10 ELISA Kit

For research use only.
Product details
Assay Type Sandwich-ELISA
Analyte IL-10
Format 96T
Reactivity Human
Regulatory Status RUO
Sensitivity <2.0 pg/mL
Standard Curve Range 4.11 pg/mL-1000 pg/mL
Assay Time 2 hr
Suitable Sample Type For the quantitative determination of human IL-10 in Plasma and Serum.
Sample volume 50 μL
Materials provided
IDComponentsSize
CEA005-C01Pre-coated Anti-IL-10 Antibody Microplate1 plate
CEA005-C02IL-10 Calibrator60 μg×2
CEA005-C03Biotin-Anti-IL-10 Antibody Concentrated Solution100 μL
CEA005-C04Biotin Antibody Dilution Buffer8 mL
CEA005-C05IL-10 Streptavidin-HRP Concentrated Solution0.5 mL
CEA005-C06Streptavidin-HRP Dilution Buffer15 mL
CEA005-C0720× Washing Buffer50 mL
CEA005-C08Sample Dilution Buffer15 mL×2
CEA005-C09Substrate Solution12 mL
CEA005-C10Stop Solution6 mL
  • Background
    ClinMax™ Human IL-10 Quantitative ELISA Kit is designed to quantitate human IL-10 that is present in complex biological matrices, such as human serum, plasma, or buffered solution. Results are obtained by Log-Log Linear regression equation are used to draw the standard curve and calculate the sample concentration. The verification results indicate that this kit can be used for the quantitative determination of natural and recombinant human Interleukin 10 concentrations.

    ClinMax product are manufactured in a dedicated GMP facility and are compliant with our ISO 13485/9001 quality management system. Each manufactured lot of the ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation in DS.

  • Application
    This immunoassay is designed to quantitate human IL-10 that is present in human serum, plasma, buffered solution. It has been calibrated against a highly purified human IL-10 and is evaluated with standard from NIBSC/WHO. Reference Reagent Interleukin-10 (Human, rDNA derived) NIBSC code: 93/722. ACROBiosystems ClinMax™ series bioanalytical product provides you with reliable analytical solution in drug development, especially at clinical trail stage. This product series are developed and manufactured by Acro Diagnostics Inc. following the Quality Management System of ISO13485/9001.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit is used to measure the concentrations of human Interleukin 10 (IL-10) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- IL-10 Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-IL-10 Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interleukin 10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
Typical Data
 IL-10 ELISA

For each experiment, each ELISA plate needs to set the standard curve. The following CEA-C005 standard curve data is for reference only.

  • Background: IL-10
    Interleukin 10, IL-10
    IL-10 is a highly potent cytokine produced by a variety of cells. It regulates cell growth and differentiation, participates in inflammatory reactions, and immune responses, and is currently recognized as inflammation and immunosuppressive factor. It plays an important role in autoimmune diseases and inflammatory diseases.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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