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Your Position: Home > Non SARS-Cov-2 Kits > IL-2 > CEA-C008

ClinMax™ Human IL-2 ELISA Kit

For research use only.
Product details
Assay Type Sandwich-ELISA
Analyte IL-2
Format 96T
Reactivity Human
Regulatory Status RUO
Sensitivity <4.0 pg/mL
Standard Curve Range 6.25 pg/mL-800 pg/mL
Assay Time 2 hr
Suitable Sample Type For the quantitative determination of human IL-2 in Plasma and Serum.
Sample volume 50 μL
Materials provided
IDComponentsSize
CEA008-C01Pre-coated Anti-IL-2 Antibody Microplate1 plate
CEA008-C02IL-2 Calibrator5 μg×2
CEA008-C03Biotin-Anti-IL-2 Antibody Concentrated Solution100 μL
CEA008-C04Biotin Antibody Dilution Buffer8 mL
CEA008-C05IL-2 Streptavidin-HRP Concentrated Solution0.5 mL
CEA008-C06Streptavidin-HRP Dilution Buffer15 mL
CEA008-C0720× Washing Buffer50 mL
CEA008-C08Sample Dilution Buffer15 mL×2
CEA008-C09Substrate Solution12 mL
CEA008-C10Stop Solution6 mL
  • Background
    ClinMax™ Human IL-2 Quantitative ELISA Kit is a one-step quick immunoassay designed to quantitate human IL-2 that is present in complex biological matrices, such as human serum, plasma, buffered solution. This assay kit is a standard sandwich-ELISA format.

    ClinMax product are manufactured in a dedicated GMP facility and are compliant with our ISO 13485 quality management system.

    The verification scheme was designed in accordance with the 9012 "Guidelines for the Verification of Quantitative Analysis Methods of Biological Samples" in the Chinese Pharmacopoeia 2020 Edition and the ICH M10 Guideline. Sensitivity, specificity, precision, recovery, and linearity were verified for multi-batch products and multiple concentration samples. See manual for more information on validation in DS.

  • Application
    Results are obtained by Log-Log Linear regression equation are used to draw the standard curve and calculate the sample concentration. The verification results indicate that this kit can be used for the quantitative determination of natural and recombinant human IL-2 concentrations. It has been calibrated against a highly purified human IL-2 and is evaluated with standard from NIBSC/WHO. Reference Reagent Interleukin-2(Human, rDNA derived) NIBSC code: 86/500.

    It is for research use only.

  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
     IL-2 Assay Principles
Typical Data
 IL-2 ELISA

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CEA-C008 is less than 4.0 pg/mL.

Validation
Dilution Linearity

Three human serum samples with high concentrations of IL-2 were diluted 1:2, 1:4, 1:8 with Dilution Buffer to produce samples with values within the dynamic range and then assayed. On average, 97.38% of IL-2 was detected from serum samples.

 IL-2 DILUTION LINEARITY
Intra-Assay Statistics

Ten replicates of each of five samples containing different IL-2 concentrations were tested in one assay, Intra-Assay Precision CV<10%.

 IL-2 INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Five samples containing different concentrations of IL-2 were tested in three independent assays, Inter-Assay Precision CV<15%.

 IL-2 INTER-ASSAY STATISTICS
Recovery

Recombinant IL-2 was spiked into 5 human serum samples, and then analyzed. On average, 97.38% of IL-2 was recovered from serum samples.

 IL-2 RECOVERY
Interference effect

Bilirubin (simulated jaundice) concentration should be less than 20mg/dL, hemoglobin (simulated hemolysis) concentration should not be higher than 3500mg/dL, triglyceride (simulated lipemia) concentration may not be higher than 2.0g/L, Heparin concentration should be less than 40U/mL, EDTA concentration should be less than 4mg/mL, and Sodium citrate concentration may not be higher than 40mg/mL, it does not affect the detection result.

 IL-2 INTERFERENCE EFFECT
  • Background: IL-2
    Interleukin 2, IL-2
    IL-2 is produced by activated T cells and promotes further growth and differentiation of activated T cells. It is a 15.5–16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the bodys natural response to microbial infection, and in discriminating between foreign ("non-self") and "self".Its effects include the proliferation of CTL, T-helper cells, and NK cells, while enhancing the secretion of IFN-γ and TNF-α.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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