|Standard Curve Range||19.5 pg/mL-1250 pg/mL|
|Assay Time||2 hr 50 min|
|Suitable Sample Type||For the quantitative determination of human TNF-α in Cell Culture Supernatants, Plasma, Serum.|
|Sample volume||100 μL|
|CRS002-C01||Pre-coated Anti-TNF-α Antibody Microplate||1plate|
|CRS002-C02||Human TNF-α Standard||20μg|
|CRS002-C05||10xWashing Buffer||50 mL|
|CRS002-C06||2xDilution Buffer||50 mL|
|CRS002-C07||Substrate Solution||12 mL|
|CRS002-C08||Stop Solution||7 mL|
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 6 simple steps:
a) All reagents were returned to room temperature(20°C-25°C) before use.
b) Add your sample to the plate, take the Human TNF-α as standard sample. The samples and standard sample are diluted by Dilution Buffer.
c) Wash the plate and add a diluted Biotin-Anti-TNF-α Antibody to the plate. The diluted by Dilution Buffer.
d) Wash the plate and add a diluted Streptavidin-HRP to the plate. The diluted by Dilution Buffer.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
For each experiment, each ELISA plate needs to set the standard curve. The following CRS-A002 standard curve data is for reference only.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $280.00
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