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Your Position: Home > Kits > TNF-alpha > CRS-A002

ClinMax™ Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit

For research use only.

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Product Details
Assay TypeSandwich-ELISA
AnalyteTNF-α
Format96T
ReactivityHuman
Regulatory StatusRUO
Sensitivity2.321 pg/mL
Standard Curve Range19.5 pg/mL-1250 pg/mL
Assay Time2 hr 50 min
Suitable Sample TypeFor the quantitative determination of human TNF-α in Cell Culture Supernatants, Plasma, Serum.
Sample volume100 μL
Materials Provided
IDComponentsSize
CRS002-C01Pre-coated Anti-TNF-α Antibody Microplate1plate
CRS002-C02Human TNF-α Standard20μg
CRS002-C03Biotin-Anti-TNF-α Antibody20μg
CRS002-C04Streptavidin-HRP50 μL
CRS002-C0510xWashing Buffer 50 mL
CRS002-C062xDilution Buffer50 mL
CRS002-C07Substrate Solution12 mL
CRS002-C08Stop Solution7 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human TNF-alpha that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of TNF-α in human serum and cell culture supernates.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage & Shipping
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.

    Below is the Shipping Statement for Kit Products.

    Shipping Statement

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human TNF-α. The kit consists of Pre-coated Anti-TNF-α Antibody Microplate and Human TNF-α Standard and Biotin-Anti-TNF-α Antibody and Streptavidin-HRP and buffers.

    Your experiment will include 6 simple steps:

    a) Bring all reagents to room temperature(20°C-25°C) before use.

    b) Add your sample to the plate and take the Human TNF-α as standard. The samples and standard are diluted by Dilution Buffer.

    c) Wash the plate and add the Biotin-Anti-TNF-α Antibody diluted by Dilution Buffer to the plate.

    d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.

    e) Wash the plate and add TMB.

    f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.

Typical Data Please refer to DS document for The assay protocol.
 TNF-alpha TYPICAL DATA

For each experiment, each ELISA plate needs to set the standard curve. The following CRS-A002 standard curve data is for reference only.

  • Background: TNF-alpha
    DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα
    Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
  • Clinical and Translational Updates

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