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Your Position: Home > Non SARS-Cov-2 Kits > TNF-alpha > CRS-A002

Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit

For research use only.
Product details
Assay Type Sandwich-ELISA
Analyte TNF-α
Format 96T
Reactivity Human
Regulatory Status RUO
Sensitivity 2.321 pg/mL
Standard Curve Range 19.5 pg/mL-1250 pg/mL
Assay Time 2 hr 50 min
Suitable Sample Type For the quantitative determination of human TNF-α in Cell Culture Supernatants, Plasma, Serum.
Sample volume 100 μL
Materials provided
IDComponentsSize
CRS002-C01Pre-coated Anti-TNF-α Antibody Microplate1plate
CRS002-C02Human TNF-α Standard20μg
CRS002-C03Biotin-Anti-TNF-α Antibody20μg
CRS002-C04Streptavidin-HRP50 μL
CRS002-C0510xWashing Buffer 50 mL
CRS002-C062xDilution Buffer50 mL
CRS002-C07Substrate Solution12 mL
CRS002-C08Stop Solution7 mL
  • Background
    Tumour Necrosis Factor alpha (TNF-α) ELISA Kit is based on ELISA sandwich method and designed to measure human TNF-α levels in cell culture supernates, serum, and plasma. It contains recombinant human TNF-α and a pair of antibodies against the recombinant factor, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of natural and recombinant human TNF-α concentrations. The specificity has been verified.

  • Application
    The kit is developed for quantitative detection of TNF-α in human serum and cell culture supernates.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human TNF-α. The kit consists of Pre-coated Anti-TNF-α Antibody Microplate and Human TNF-α Standard and Biotin-Anti-TNF-α Antibody and Streptavidin-HRP and buffers.

    Your experiment will include 6 simple steps:

    a) All reagents were returned to room temperature(20°C-25°C) before use.

    b) Add your sample to the plate, take the Human TNF-α as standard sample. The samples and standard sample are diluted by Dilution Buffer.

    c) Wash the plate and add a diluted Biotin-Anti-TNF-α Antibody to the plate. The diluted by Dilution Buffer.

    d) Wash the plate and add a diluted Streptavidin-HRP to the plate. The diluted by Dilution Buffer.

    e) Wash the plate and add TMB.

    f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data
 TNF-alpha ELISA

For each experiment, each ELISA plate needs to set the standard curve. The following CRS-A002 standard curve data is for reference only.

  • Background: TNF-alpha
    DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα
    Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimers disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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