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Your Position: Home > CAR-T > HLA-A*11:01 & B2M > HLM-H82W5

Biotinylated Human HLA-A*11:01&B2M Complex Protein (Peptide free, MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    HLA-A*11:01 & B2M
  • Source
    Biotinylated Human HLA-A*11:01&B2M Complex Protein(HLM-H82W5) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) (Accession # AAV53343.1 (HLA-A*11:01) & P61769 (B2M)).
    Predicted N-terminus: Gly 25 & Ile 21
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 36.0 kDa and 11.7 kDa. The protein migrates as 42-45 kDa and 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-A*11:01 & B2M SDS-PAGE

Biotinylated Human HLA-A*11:01&B2M Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
HLA-A*11:01 & B2M MALS images

The purity of Biotinylated Human HLA-A*11:01&B2M Complex Protein (Cat. No. HLM-H82W5) is more than 90% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.

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ETD of in-stock products: 2 business days

Price(USD) : $120.00

Price(USD) : $680.00

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Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

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