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Your Position: Home > Viral Proteins > Hemagglutinin (HA) > HA2-V82E3

Biotinylated Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) Protein, Avitag™,His Tag (MALS verified)

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  • Synonym
    HA,Hemagglutinin
  • Source
    Biotinylated Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA, Avitag,His Tag (HA2-V82E3) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Lys 523 (Accession # Q80KD9-1).
    Predicted N-terminus: Asp 19
  • Molecular Characterization
    Online(Asp 19 - Lys 523) Q80KD9-1

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 60.0 kDa. The protein migrates as 66.0 kDa under reducing (R) condition (SDS-PAGE).

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA, Avitag,His Tag (Cat. No. HA2-V82E3) SDS-PAGE gel

Biotinylated Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA, Avitag,His Tag (Cat. No. ) MALS images

The purity of Biotinylated Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA, Avitag,His Tag (Cat. No. HA2-V82E3) is more than 90% and the molecular weight of this protein is around 78-98 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA, Avitag,His TagBiotinylated Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA, Avitag,His Tag (Cat. No. HA2-V82E3) ELISA bioactivity

Immobilized Monoclonal Anti-Influenza HA Universal Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA, Avitag,His Tag (Cat. No. HA2-V82E3) with a linear range of 10-156 ng/mL (QC tested).

  • Background
    Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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