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Your Position: Home > Non SARS-Cov-2 Kits > GM-CSF > CEA-C007

ClinMax™ Human GM-CSF ELISA Kit

For research use only.
Product details
Assay Type Sandwich-ELISA
Analyte GM-CSF
Format 96T
Reactivity Human
Regulatory Status RUO
Sensitivity <3.0 pg/mL
Standard Curve Range 7.81 pg/mL-1000 pg/mL
Assay Time 2 hr
Suitable Sample Type For the quantitative determination of human GM-CSF in Plasma and Serum.
Sample volume 50 μL
Materials provided
IDComponentsSize
CEA007-C01Pre-coated Anti-GM-CSF Ab Microplate1 plate
CEA007-C02GM-CSF Calibrator20 μg×2
CEA007-C03Biotin-Anti-GM-CSF Ab Con. Solution100 μL
CEA007-C04Biotin-Ab Dilution Buffer8 mL
CEA007-C05GM-CSF SA-HRP Con. Solution0.5 mL
CEA007-C06SA-HRP Dilution Buffer15 mL
CEA007-C0720× Washing Buffer50 mL
CEA007-C08Sample Dilution Buffer15 mL×2
CEA007-C09Substrate Solution12 mL
CEA007-C10Stop Solution6 mL
  • Background
    ClinMax™ Human GM-CSF Quantitative ELISA Kit is a one-step quick immunoassay designed to quantitate human GM-CSF that is present in complex biological matrices, such as human serum, plasma, buffered solution. This assay kit is a standard sandwich-ELISA format.

    ClinMax product are manufactured in a dedicated GMP facility and are compliant with our ISO 13485 quality management system.

    The verification scheme was designed in accordance with the 9012 "Guidelines for the Verification of Quantitative Analysis Methods of Biological Samples" in the Chinese Pharmacopoeia 2020 Edition and the ICH M10 Guideline. Sensitivity, specificity, precision, recovery, and linearity were verified for multi-batch products and multiple concentration samples. See manual for more information on validation in DS.

  • Application
    Results are obtained by Log-Log Linear regression equation are used to draw the standard curve and calculate the sample concentration. The verification results indicate that this kit can be used for the quantitative determination of natural and recombinant human GM-CSF concentrations. It has been calibrated against a highly purified human GM-CSF and is evaluated with standard from NIBSC/WHO. Reference Reagent Granulocyte Macrophage Colony Stimulating Factor (Human, rDNA derived) NIBSC code: 88/646.

    It is for research use only.

  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
     GM-CSF Assay Principles
Typical Data
 GM-CSF ELISA

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CEA-C007 is less than 3.0 pg/mL.

Validation
Dilution Linearity

Three human serum samples with high concentrations of GM-CSF were diluted 1:2, 1:4, 1:8, 1:16, 1:32,1:64 with Dilution Buffer to produce samples with values within the dynamic range and then assayed. On average, 94.18% of GM-CSF was detected from serum samples.

 GM-CSF DILUTION LINEARITY
Intra-Assay Statistics

Ten replicates of each of five samples containing different GM-CSF concentrations were tested in one assay , Intra-Assay Precision CV<10%.

 GM-CSF INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Five samples containing different concentrations of GM-CSF were tested in three independent assays , Inter-Assay Precision CV<15%.

 GM-CSF INTER-ASSAY STATISTICS
Recovery

Recombinant GM-CSF was spiked into 5 human serum samples, and then analyzed. On average, 102.60% of GM-CSF was recovered from serum samples.

 GM-CSF RECOVERY

Recombinant GM-CSF was spiked into cell supernatant sample, and then analyzed. On average, 97.73% of GM-CSF was recovered from serum samples.

 GM-CSF RECOVERY
Interference effect

Bilirubin (simulated jaundice) concentration should be less than 20mg/dL, hemoglobin (simulated hemolysis) concentration should not be higher than 3500mg/dL, triglyceride (simulated lipemia) concentration may not be higher than 2.0g/L, Heparin concentration should be less than 40U/mL, EDTA concentration should be less than 4mg/mL, and Sodium citrate concentration may not be higher than 40mg/mL, it does not affect the detection result.

 GM-CSF INTERFERENCE EFFECT

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