|EP130-C03||Biotinylated Human PD-L1||20μg|
|EP130-C04||Anti-PD-1 Neutralizing Antibody||20μg|
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
Your experiment will include 4 simple steps:
a) Coat the plate with human PD-1.
b) Add your molecule of interest to the tests.
c) Add human PD-L1-Biotin to bind the coated human PD-1.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to PD-1: PD-L1 binding will be determined by comparing OD readings among different experimental groups.
PD-1: PD-L1 [BIOTINYLATED] BINDING IN THE ABSENCE OF INHIBITORS
Immobilized human PD-1 protein at 0.4μg/mL (100μL/well) can bind biotinylated human PD-L1 with a linear range of 0.01-5μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points before curve fitting (QC tested).
INHIBITION OF PD-1: PD-L1 [BIOTINYLATED] BINDING BY ANTI-BAFFR NEUTRALIZING ANTIBODY
Serial dilutions of Anti-PD-1 Neutralizing antibody (Catalog # EP130-C04) (1:1 serial dilution, from 5μg/mL to 0.02μg/mL) was added into PD-1: Biotinylated PD-L1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Price(USD) : $600.00
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