|EP121-C01||High-bind Plate||1 plate|
|EP121-C02||Human CD27||20 μg|
|EP121-C03||Biotinylated Human CD27L||10 μg|
|EP121-C04||Anti-CD27L Neutralizing Antibody||20 μg|
|EP121-C06||Coating Buffer||12 mL|
|EP121-C07||10xWashing Buffer||50 mL|
|EP121-C08||Blocking Buffer||50 mL|
|EP121-C09||Substrate Solution||12 mL|
|EP121-C10||Stop Solution||7 mL|
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human CD27.
b) Add your molecule of interest to the tests.
c) Add human CD27L-Biotin to bind the coated human CD27.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to CD27L: CD27 binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF CD27L [BIOTINYLATED]: CD27 BINDING BY ANTI-CD27L NEUTRALIZING ANTIBODY
Serial dilutions of Anti-CD27L Neutralizing antibody (Catalog # EP121-C04) (1:1 serial dilution, from 5 μg/mL to 0.005μg/mL) was added into CD27: Biotinlated CD27L binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $650.00
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