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Your Position: Home > Non SARS-Cov-2 Kits > CD27 > EP-121

CD27L [Biotinylated] : CD27 Inhibitor Screening ELISA Kit

For research use only.
IDComponentsSize
EP121-C01High-bind Plate1 plate
EP121-C02Human CD2720 μg
EP121-C03Biotinylated Human CD27L10 μg
EP121-C04Anti-CD27L Neutralizing Antibody20 μg
EP121-C05Streptavidin-HRP10 μg
EP121-C06Coating Buffer12 mL
EP121-C0710xWashing Buffer 50 mL
EP121-C08Blocking Buffer50 mL
EP121-C09Substrate Solution12 mL
EP121-C10Stop Solution7 mL
  • Background
    Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.
  • Application
    This kit is developed for screening for inhibitors of human CD27 binding to human CD27L

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new CD27L pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human CD27L to immobilized human CD27 in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinlated CD27L protein, a human CD27 protein, an anti-CD27L neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

    Your experiment will include 4 simple steps:

    a) Coat the plate with human CD27.

    b) Add your molecule of interest to the tests.

    c) Add human CD27L-Biotin to bind the coated human CD27.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the half maximal inhibitory concentration (IC50) of your compound to CD27L: CD27 binding will be determined by comparing OD readings among different experimental groups.

Typical Data
 CD27 ELISA

INHIBITION OF CD27L [BIOTINYLATED]: CD27 BINDING BY ANTI-CD27L NEUTRALIZING ANTIBODY
Serial dilutions of Anti-CD27L Neutralizing antibody (Catalog # EP121-C04) (1:1 serial dilution, from 5 μg/mL to 0.005μg/mL) was added into CD27: Biotinlated CD27L binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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Price(USD) : $650.00

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