|EP120-C01||High-bind Plate||1 plate|
|EP120-C02||Human PD-L2||50 μg|
|EP120-C03||Biotinylated Human PD-1||10 μg|
|EP120-C04||Anti-PD-1 Neutralizing Antibody||20 μg|
|EP120-C06||Coating Buffer||12 mL|
|EP120-C07||10xWashing Buffer||50 mL|
|EP120-C08||Blocking Buffer||50 mL|
|EP120-C09||Substrate Solution||24 mL|
|EP120-C10||Stop Solution||7 mL|
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human PD-L2.
b) Add your molecule of interest to the tests.
c) Add human PD-1-Biotin to bind the coated human PD-L2.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to PD-L2: PD-1 binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF PD-1 [BIOTINYLATED]: PD-L2 BINDING BY ANTI-PD-1 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-PD-1 Neutralizing antibody (Catalog # EP120-C04) (1:1 serial dilution, from 5 μg/mL to 0.01μg/mL) was added into PD-L2: Biotinlated PD-1binding reactions. The assay was performed according to the protocol described above. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $650.00
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