|EP120-C01||High-bind Plate||1 plate|
|EP120-C02||Human PD-L2||50 μg|
|EP120-C03||Biotinylated Human PD-1||10 μg|
|EP120-C04||Anti-PD-1 Neutralizing Antibody||20 μg|
|EP120-C06||Coating Buffer||12 mL|
|EP120-C07||10xWashing Buffer||50 mL|
|EP120-C08||Blocking Buffer||50 mL|
|EP120-C09||Substrate Solution||24 mL|
|EP120-C10||Stop Solution||7 mL|
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human PD-L2.
b) Add your molecule of interest to the tests.
c) Add human PD-1-Biotin to bind the coated human PD-L2.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to PD-L2: PD-1 binding will be determined by comparing OD readings among different experimental groups.
PD-1 [BIOTINYLATED]: PD-L2 BINDING IN THE ABSENCE OF INHIBITORS
Immobilized human PD-L2 protein at 4 μg/mL (100 μL/well) can bind biotinylated human PD-1 with a linear range of 0.00078-0.4μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points before curve fitting (QC tested).
INHIBITION OF PD-1 [BIOTINYLATED]: PD-L2 BINDING BY ANTI-PD-1 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-PD-1 Neutralizing antibody (Catalog # EP120-C04) (1:1 serial dilution, from 5 μg/mL to 0.01μg/mL) was added into PD-L2: Biotinlated PD-1binding reactions. The assay was performed according to the protocol described above. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Price(USD) : $650.00
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