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| ID | Components | Size |
| EP129-C01 | High-bind Plate | 1 plate |
| EP129-C02 | Human IL-7 R alpha & TSLP R | 25 μg |
| EP129-C03 | Anti- TSLP Neutralizing Antibody | 20 μg |
| EP129-C04 | Human TSLP-Biotin | 10 μg |
| EP129-C05 | Streptavidin-HRP | 10 μg |
| EP129-C06 | Coating Buffer | 12 mL |
| EP129-C07 | 10xWashing Buffer | 50 mL |
| EP129-C08 | Blocking Buffer | 50 mL |
| EP129-C09 | Substrate Solution | 12 mL |
| EP129-C10 | Stop Solution | 7 mL |
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-7 R alpha & TSLP R.
b) Add your molecule of interest to the tests.
c) Add human TLSP-Biotin to bind the coated human IL-7 R alpha & TSLP R.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit TSLP: IL-7 R alpha & TSLP Rbinding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF TSLP [BIOTINYLATED]: IL-7 R ALPHA & TSLP R BINDING BY ANTI-TSLP NEUTRALIZING ANTIBODY
Serial dilutions of Anti-TSLP Neutralizing antibody (Catalog # EP129-C03) (1:1 serial dilution, from 8 μg/mL to 0.0156 μg/mL (55.317-0.108 nM)) was added into IL-7 R alpha & TSLP R: TSLP-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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