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| Assay Type | Sandwich-ELISA |
| Analyte | Nuclease |
| Format | 96T |
| Regulatory Status | RUO |
| Sensitivity | < 39 pg/mL |
| Standard Curve Range | 39.06 pg/mL-2500 pg/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the quantitative determination the residue of nuclease in biologicals. |
| Sample volume | 100 μL |
| ID | Components | Size |
| CRS016-C01 | Pre-coated Anti-Nuclease Antibody Microplate | 1 plate |
| CRS016-C02 | Nuclease Standard | 50 μL |
| CRS016-C03 | Biotin-Anti-Nuclease Antibody | 100 μL |
| CRS016-C04 | Streptavidin-HRP | 50 μL |
| CRS016-C05 | 10xWashing Buffer | 50 mL |
| CRS016-C06 | 2xDilution Buffer | 50 mL |
| CRS016-C07 | Substrate Solution | 12 mL |
| CRS016-C08 | Stop Solution | 7 mL |
The GENIUS™ELISA Kit was developed for the detection and quantitative determination of nuclease in samples from downstream processing where nuclease is used as a process or purification aid.
The limitation of detectable dose (LOD) of Cat. No. CRS-A016 was determined as 2.733 pg/mL.
It is for research use only.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.
Below is the Shipping Statement for Kit Products.
Your experiment will include 6 simple steps:
a) All reagents were returned to room temperature(20°C-25°C) before use.
b) Add your sample to the plate, take the Nuclease as standard sample. The samples and standard sample are diluted by Dilution Buffer.
c) Add a diluted Biotin-Anti-Nuclease Antibody to the plate. The diluted by Dilution Buffer.
d) Wash the plate and add a diluted Streptavidin-HRP to the plate. The diluted by Dilution Buffer.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
GENIUS™ Nuclease ELISA Kit (Residue Testing) (Cat. No. CRS-A016) can detect the nuclease from different manufacturers with similar sensitivity.
GENIUS™ Nuclease ELISA Kit (Residue Testing) (Cat. No. CRS-A016) can detect the nuclease in different culture supernatant with similar curve and sensitivity.
GENIUS™ Nuclease ELISA Kit (Residue Testing) (Cat. No. CRS-A016) can detect the nuclease from different culture supernatant and was free from the matrix effects. * means a significant difference compared with the negative, NS means no significant difference with the negative.
Intra-Assay Precision : Twenty replicates of each of three samples containing different concentrations were tested in one assay. Within the detection range, the CV is less than 10%.
Inter-Assay Precision : Three replicates of each of three samples containing different concentrations were tested in three independent assays. Within the detection range, the CV is less than 10%.
Three parts of blank medium were added with three different concentrations of Nuclease, and the medium without Nuclease was used as background to calculate the recovery rate.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $650.00
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