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Your Position: Home > CKP > Streptavidin > STN-NA114

Streptavidin-Acridine ester (MALS verified)

  • Synonym
    Streptavidin,SA
  • Source
    Streptavidin-Acridine ester (STN-NA114) is Acridine ester chemically conjugated Streptavidin expressed from E.coli cells.
  • Molecular Characterization

    Streptavidin carries no "tag". The protein has a calculated MW of 13.8 kDa. The protein migrates as 15-16 kDa under reducing (R) condition (SDS-PAGE).

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Streptavidin SDS-PAGE

Streptavidin-Acridine ester on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Streptavidin MALS images

The purity of Streptavidin-Acridine ester(Cat. No. STN-NA114) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.

  • Background
    Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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