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Your Position: Home > Protein > Streptavidin > STN-NA114

Streptavidin Protein-Acridinium ester (MALS verified)

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  • Synonym
    Streptavidin,SA
  • Source
    Streptavidin Protein-Acridinium ester (STN-NA114) is Acridinium ester chemically conjugated Streptavidin expressed from E. coli cells.
  • Molecular Characterization

    Streptavidin carries no "tag". The protein has a calculated MW of 13.8 kDa. The protein migrates as 15-16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Labeling
    Acridinium ester, can react with the primary amino group of protein. Under alkaline conditions, NHS is replaced as the leaving group, and the protein forms a stable amide bond with Acridinium ester.
  • Protein Ratio
    Passed as determined by binding MPCLIA.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Streptavidin SDS-PAGE

Streptavidin Protein-Acridinium ester on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Streptavidin MALS images

The purity of Streptavidin Protein-Acridinium ester (Cat. No. STN-NA114) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.

Bioactivity-MPCLIA
 Streptavidin MPCLIA

Immobilized 0.04 μg/Test of Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4) to the Streptavidin Protein-Acridinium ester (Cat. No. STN-NA114, 0.008 μg/Test), incubated with 20 μL/Test of Human PD-L1 Protein, Mouse IgG1 Fc Tag (Cat. No. PD1-H52A3) at increasing concentration coupled to Anti-Mouse IgG Magnetic Beads (10 μg beads/Test). Detection was performed with sensitivity of 7.8 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).

 Streptavidin MPCLIA

Immobilized 0.04 μg/Test of Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82E9) to the Streptavidin Protein-Acridinium ester (Cat. No. STN-NA114, 0.008 μg/Test), incubated with 20 μL/Test of Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (Cat. No. S1N-M12A1) at increasing concentration coupled to Protein G Magnetic Beads (10 μg beads/Test). Detection was performed with sensitivity of 0.244 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (Routinely tested).

 Streptavidin MPCLIA

Immobilized 0.04 μg/Test of Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82E9) to the Streptavidin Protein-Acridinium ester (Cat. No. STN-NA114, 0.008 μg/Test), incubated with 20 μL/Test of Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (Cat. No. S1N-M12A1) at increasing concentration coupled to Anti-Human IgG Magnetic Beads (10 μg beads/Test). Detection was performed with sensitivity of 7.8 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (Routinely tested).

  • Background
    Streptavidin is a 66KDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity to biotin. Each unit can bind one biotin. Horseradish peroxidase is metalloenzyme, a 44KDa glycoprotein. When incubate with substrates, it produces a coloured, fluorimetric, or luminescent derivatives, which can be detected and quantified. HRP conjugated Streptavidin is widely used for the detection and quantification of biotinylated proteins.
  • Clinical and Translational Updates

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