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Your Position: Home > Others > IL-7 > CRS-A006

Human Interleukin-7 (IL-7) ELISA Kit (Residue Testing)

For research use only.
Product Details
Assay Type Sandwich-ELISA
Analyte IL-7
Format 96T
Reactivity Human
Regulatory Status RUO
Sensitivity 8.223pg/mL
Standard Curve Range 31.3 pg/mL-2000 pg/mL
Assay Time 2 hr 50 min
Suitable Sample Type For the quantitative determination of human IL-7 in Cell Culture Supernatants, Plasma, Serum.
Sample volume 100 μL
Materials Provided
CRS006-C01Pre-coated Anti-IL-7 Antibody Microplate1 plate
CRS006-C02Human IL-7 Standard20 μg
CRS006-C03Biotin-Anti-IL-7 Antibody100 μL
CRS006-C04Streptavidin-HRP50 μL
CRS006-C0510xWashing Buffer 50 mL
CRS006-C062xDilution Buffer50 mL
CRS006-C07Substrate Solution12 mL
CRS006-C08Stop Solution7 mL
  • Background
    Human Interleukin-7 (IL-7) ELISA Kit (Residue Testing) is based on ELISA sandwich method and designed to detect and quantitative determine of GMP human IL-7 in samples from CAR-T product preparation processing. It contains GMP human IL-7 and a pair of antibodies against the recombinant factor, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of GMP human IL-7 (ACROBiosystems, cat#GMP-L07H24) concentrations. The specificity has been verified.
  • Application

    The Human Interleukin-7 (IL-7) ELISA Kit was developed for the detection and quantitative determination of GMP human IL-7 in samples from CAR-T product preparation processing where IL-7 is used as an immunotherapy agent.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage & Shipping
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.

    Below is the Shipping Statement for Kit Products.

    Shipping Statement

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Nuclease. The kit consists of Pre-coated Anti-IL-7 Antibody Microplate and Human IL-7 Standard and Biotin-Anti-IL-7 Antibody and Streptavidin-HRP and buffers.

    Your experiment will include 6 simple steps:

    a) All reagents were returned to room temperature(20°C-25°C) before use.

    b) Add your sample to the plate, take the Human IL-7 as standard sample. The samples and standard sample are diluted by Dilution Buffer.

    c) Add a diluted Biotin-Anti-IL-7 Antibody to the plate. The diluted by Dilution Buffer.

    d) Wash the plate and add a diluted Streptavidin-HRP to the plate. The diluted by Dilution Buffer.

    e) Wash the plate and add TMB.

    f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data Please refer to DS document for The assay protocol.

For each experiment, each ELISA plate needs to set the standard curve. The following CRS-A006 standard curve data is for reference only.

  • Background: IL-7
    IL7, Interleukin-7
    Interleukin 7 is also known as IL7, IL-7, and is a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells, but is not produced by lymphocytes. IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells, It also stimulates proliferation of all cells in the lymphoid lineage (B cells, T cells and NK cells). It is important for proliferation during certain stages of B-cell maturation, T and NK cell survival, development and homeostasis. IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL-7 binds to the IL-7 receptor, a heterodimer consisting of Interleukin-7 receptor alpha and common gamma chain receptor. IL-7 promotes hematological malignacies (acute lymphoblastic leukemia, T cell lymphoma). Elevated levels of IL-7 have also been detected in the plasma of HIV-infected patients. IL-7 as an immunotherapy agent has been examined in many pre-clinical animal studies and more recently in human clinical trials for various malignancies and during HIV infection. IL-7 could also be beneficial in improving immune recovery after allogenic stem cell transplant.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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