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Your Position: Home > Non SARS-Cov-2 Kits > IL-15 > CRS-A009

Human Interleukin-15(IL-15) ELISA Kit

For research use only.
Product details
Assay Type Sandwich-ELISA
Analyte IL-15
Format 96T
Reactivity Human
Regulatory Status RUO
Sensitivity 2.282 pg/mL
Standard Curve Range 4.7 pg/mL-300 pg/mL
Assay Time 2 hr 50 min
Suitable Sample Type For the quantitative determination of human IL-15 in Cell Culture Supernatants, Plasma, Serum.
Sample volume 100 μL
Materials provided
IDComponentsSize
CRS009-C01Pre-coated Anti-IL-15 Antibody Microplate1 plate
CRS009-C02Human IL-15 Standard20 μg
CRS009-C03Biotin-Anti-IL-15 Antibody15 μg
CRS009-C04Streptavidin-HRP50 μL
CRS009-C0510xWashing Buffer 50 mL
CRS009-C062xDilution Buffer50 mL
CRS009-C07Substrate Solution12 mL
CRS009-C08Stop Solution7 mL
  • Background
    Interleukin-15 (IL-15) ELISA Kit is based on ELISA sandwich method and designed to measure human IL-15 levels in cell culture supernates, serum, and plasma. It contains recombinant human IL-15 and a pair of antibodies against the recombinant factor, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of natural and recombinant human IL-15 concentrations. The specificity has been verified.
  • Application
    The kit is developed for quantitative detection of IL-15 in human serum and cell culture supernates.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage & Shipping
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.

    Below is the Shipping Statement for Kit Products.

    Shipping Statement

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human IL-15. The kit consists of Pre-coated Anti-IL-15 Antibody Microplate and Human IL-15 Standard and Biotin-Anti-IL-15 Antibody and Streptavidin-HRP and buffers.

    Your experiment will include 6 simple steps:

    a) All reagents were returned to room temperature(20°C-25°C) before use.

    b) Add your sample to the plate, take the Human IL-15 as standard sample. The samples and standard sample are diluted by Dilution Buffer.

    c) Wash the plate and add a diluted Biotin-Anti-IL-15 Antibody to the plate. The diluted by Dilution Buffer.

    d) Wash the plate and add a diluted Streptavidin-HRP to the plate. The diluted by Dilution Buffer.

    e) Wash the plate and add TMB.

    f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data Please refer to DS document for The assay protocol.
 IL-15 ELISA

For each experiment, each ELISA plate needs to set the standard curve. The following CRS-A009 standard curve data is for reference only.

  • Background: IL-15
    IL-15, Interleukin-15, MGC9721
    Interleukin 15 is also known as IL15, IL-15, and is a cytokine with structural similarity to IL-2. Like IL-2, IL-15 binds to and signals through the IL-2/IL-15 beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). This cytokine induces cell proliferation of natural killer cells; cells of the innate immune system whose principal role is to kill virally infected cells. Interleukin 15 (IL-15) regulates T and natural killer (NK) cell activation and proliferation. Survival signals that maintain memory T cells in the absence of antigen are provided by IL-15. This cytokine is also implicated in NK cell development. In rodent lymphocytes, IL-15 prevents apoptosis by inducing an apoptosis inhibitor, BCL2L1/BCL-x(L). IL-15 has been shown to enhance the anti-tumor immunity of CD8+ T cells in pre-clinical models. A phase I clinical trial to evaluate the safety, dosing, and anti-tumor efficacy of IL-15 in patients with metastatic melanoma and renal cell carcinoma (kidney cancer) has begun to enroll patients at the National Institutes of Health.

  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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