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Your Position: Home > CAR-T > HLA-A*0201 & B2M & GP100 > HLG-H82E6

Biotinylated Human HLA-A*0201 GP100 (YLEPGPVTA) Complex Protein (Monomer, MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    HLA-A*0201 & B2M & GP100
  • Source
    Biotinylated Human HLA-A*0201 GP100 (YLEPGPVTA) Complex Protein (HLG-H82E6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*0201) & YLEPGPVTA & Ile 21 - Met 119 (B2M) (Accession # A0A140T913(HLA-A*0201) & P61769(B2M)).
    Predicted N-terminus: Gly 25 & Tyr
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 36.0 kDa and 13.7 kDa. The protein migrates as 36-40 kDa, 17 kDa and 15 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human HLA-A*0201 GP100 (YLEPGPVTA) Complex Protein (Cat. No. HLG-H82E6) SDS-PAGE gel

Biotinylated Human HLA-A*0201 GP100 (YLEPGPVTA) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human HLA-A*0201 GP100 (YLEPGPVTA) Complex Protein (Cat. No. ) MALS images

The purity of Biotinylated Human HLA-A*0201 GP100 (YLEPGPVTA) Complex Protein (Cat. No. HLG-H82E6) is more than 95% and the molecular weight of this protein is around 39-59 kDa verified by SEC-MALS.

  • Background
    Glycoprotein 100, is also well known as gp100 and Melanocyte protein PMEL. This antigen is a melanocyte-specific type I transmembrane glycoprotein and composed of 661 amino acids. Glycoprotein 100 is related to the tumor regression in metastatic melanoma patients after adoptive therapy, by which its epitopes being recognized by tumor lymphocytes and T-cells. The gp100280–288(YLEPGPVTA) was originally shown to be recognized by HLA-A*0201 tumor-infiltrating lymphocytes from melanoma patients, and therefore it is widely been studied in TCR -T studies.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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