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Your Position: Home > CAR-T > HLA-A*02:01 & B2M & Glycipan 3 > HLG-H82E4

Biotinylated Human HLA-A*02:01&B2M&Glycipan 3 (FVGEFFTDV) Complex Protein (Monomer, MALS verified)

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  • Synonym
    HLA-A*02:01 & B2M & Glycipan 3
  • Source
    Biotinylated Human HLA-A*02:01&B2M&Glycipan 3 (FVGEFFTDV) Complex Protein(HLG-H82E4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & FVGEFFTDV peptide (Accession # A0A140T913 (HLA-A*02:01) & P61769 (B2M) & FVGEFFTDV ).
    Predicted N-terminus: Gly 25 & Phe
  • Molecular Characterization

    Biotinylated Human HLA-A*02:01&B2M&Glycipan 3 (FVGEFFTDV) Complex Protein is produced by co-expression of HLA and B2M loaded with Glycipan 3 peptide.

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 36.0 kDa and 13.8 kDa. The protein migrates as 42-45 kDa and 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-A*02:01 & B2M & Glycipan 3 SDS-PAGE

Biotinylated Human HLA-A*02:01&B2M&Glycipan 3 (FVGEFFTDV) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
HLA-A*02:01 & B2M & Glycipan 3 MALS images

The purity of Biotinylated Human HLA-A*02:01&B2M&Glycipan 3 (FVGEFFTDV) Complex Protein (Cat. No. HLG-H82E4) is more than 95% and the molecular weight of this protein is around 39-59 kDa verified by SEC-MALS.

  • Background
    Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. The Human HLA-A*0201 Glycipan 3 (FVGEFFTDV) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M and FVGEFFTDV peptide of the Glycipan 3 .
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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