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Your Position: Home > CKP > DNP > DNP-M486

Monoclonal Anti-DNP antibody, Mouse IgG2b Isotype Control

  • Source
    Dinitrophenyl (DNP) is a hapten that is not normally expressed in the target tissue. Therefore, anti-DNP antibody has no relevant specificity to a target antigen, and can be used as isotype control antibody to differentiate non-specific background signal from specific antibody signal in various in vitro and in vivo studies. Monoclonal Anti-DNP antibody, Mouse IgG2b Isotype Control was purified from HEK293 cell culture at ACRObiosystems, and specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.
  • Isotype
    Mouse IgG2b/kappa
  • Specificity
    This product is a specific antibody against DNP.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Monoclonal Anti-DNP antibody, Mouse IgG2b Isotype Control (Cat. No. DNP-M486) SDS-PAGE gel

Monoclonal Anti-DNP antibody, Mouse IgG2b Isotype Control on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Monoclonal Anti-DNP antibody, Mouse IgG2b Isotype ControlMonoclonal Anti-DNP antibody, Mouse IgG2b Isotype Control (Cat. No. DNP-M486) ELISA bioactivity

Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Mouse IgG2b Isotype Control (Cat. No. DNP-M486) with a linear range of 0.1-3 ng/mL (QC tested).

  • Background
    A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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