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| ID | Components | Size |
| EP118-C01 | High-bind Plate | 1 plate |
| EP118-C02 | Human BAFF | 10 μg |
| EP118-C03 | Biotinylated Human BCMA | 10 μg |
| EP118-C04 | Anti-BCMA Neutralizing Antibody | 20 μg |
| EP118-C05 | Streptavidin-HRP | 5 μg |
| EP118-C06 | Coating Buffer | 12 mL |
| EP118-C07 | 10xWashing Buffer | 50 mL |
| EP118-C08 | Blocking Buffer | 50 mL |
| EP118-C09 | Substrate Solution | 12 mL |
| EP118-C10 | Stop Solution | 7 mL |
This kit is developed for screening for inhibitors of human BCMA binding to human BAFF.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human BAFF.
b) Add your molecule of interest to the tests.
c) Add human BCMA-Biotin to bind the coated human BAFF.
d) Add Streptavidin-HRP followed by luminol .
Finally, the half maximal inhibitory concentration (IC50) of your compound to BCMA: BAFF binding will be determined by comparing RLU readings among different experimental groups.
INHIBITION OF BCMA [BIOTINYLATED]: BAFF BINDING BY ANTI-BCMA NEUTRALIZING ANTIBODY
Serial dilutions of Anti-BCMA Neutralizing antibody (Catalog # EP118-C04) (1:1 serial dilution, from 10 μg/mL to 0.0048828125μg/mL) was added into BAFF: Biotinylated BCMA binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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