Login Register
icon_bulk_orderBulk order Acrobiosystems for English
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > IgG kits > Spike RBD > FCM-B08R

SARS-CoV-2 Variants Total IgG 6-plex Panel (Flow Cytometry Multiplex Bead Assay, Non-premixed)

For research use only.
Product Details
Assay Type FACS
Analyte Wild-type
Alpha B.1.1.7
Beta B.1.351
Gamma P.1
Delta B.1.617.2
Omicron BA.1
Format 96T
Reactivity Human
Regulatory Status RUO
Sensitivity 16.56 ng/mL
18.33 ng/mL
14.64 ng/mL
16.23 ng/mL
15.96 ng/mL
12.81 ng/mL
Standard Curve Range 32.5~1040 ng/mL
Assay Time 1 hr 50 min
Suitable Sample Type human serum, human plasma
Sample volume 3 μL
Materials Provided
IDComponentsSize
FCM08-C01SARS-CoV-2 Antibody Calibrator20μg
FCM08-C02ARBD WT Beads200μL
FCM08-C02BRBD Alpha Beads200μL
FCM08-C02CRBD Beta Beads200μL
FCM08-C02DRBD Gamma Beads200μL
FCM08-C02ERBD Delta Beads200μL
FCM08-C02FRBD Omicron Beads200μL
FCM08-C03Assay Buffer40mL×2
FCM08-C045 × Wash Buffer10mL
FCM08-C05PE-Labeled Anti-human IgG Detection Antibody200μL
FCM08-C065 × PBS Buffer10mL
FCM08-C07APC Beads500μL
FCM08-C08PE Beads500μL
FCM08-C0996-Well V-bottom Plate1plate
FCM08-C1096-Well Sealing Film2pieces
  • Background
    Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide.As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans.To facilitate the mutant-related research, drug trials and vaccine development, a multiplex serological test for assessing antibody responses to mutant strains is in urgent need.
  • Application

    The kit is developed for qualitative detection of Anti-SARS-CoV-2 Variants (Alpha, Beta, Gamma, Delta, Omicron) total IgG antibody in human serum.

    It is for research use only.

  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    SARS-CoV-2 Variants Total IgG 6-plex Panel is a multiplex bead-based indirect immunofluorescence assay, utilizing flow cytometry for data acquisition and classification. Six SARS-CoV-2 Spike RBD protein fragments (WT / Alpha / Beta / Gamma / Delta / Omicron) were conjugated to 6 fluorochrome-encoded microspheres respectively, as indicated in Table 1. The Anti-SARS-CoV-2 IgG Antibody in blood specimens was bound to the RBD encapsulated 6-plex beads, and then detected by PE-conjugated anti-human IgG detection antibody, forming bead-analyte-detection antibody sandwiches. The intensity of PE fluorescence was assessed by flow cytometry at wavelength of 575 nm approximately, in proportion to the Anti-SARS-CoV-2 IgG titer in blood specimens. The intensity of APC fluorescence was applied to classify bead populations, at wavelength of 670 nm approximately.
*Optional: If there is vacuum manifold for 96-Well plates, 96-Well Filter Plate (ACROBiosystems, cat#FCM-B01M) is prepared.
Bioactivity-FACS Please refer to DS document for The assay protocol.
 Spike RBD FACS

A representative datapoint showing single bead population in P1 gate (left), SARS-CoV-2 variant immobilized beads classified by APC channel (middle, P2 ~ P7 gate), and measured fluorescence signal for each population in PE channel (right). Data was generated on BD FACSLyricTM Flow Cytometer, equipped with blue laser (488 nm) and red laser (640 nm).

 Spike RBD FACS

A 4-parameter logistic model fitting curve was plotted on SARS-CoV-2 Variants total IgG 6-plex Panel. Immobilized SARS-CoV-2 variant RBD proteins bind with a serial dilution of potent and broad antibody calibrator, ranging from 16.5 ng/ml to 4160 ng/ml (QC tested). EC50 values indicate the concentration at which 50% of calibrator were bound.

Comments (0)


ETD of in-stock products: 2 business days

Price(USD) : $1094.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America: +1 800-810-0816 (Toll Free)
Asia & Pacific: +86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message