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Your Position: Home > CD&BIOMARKER > HLA-C*07:02:01 & B2M & RYR > HLR-H52W2

Biotinylated Human HLA-C*07:02:01 & B2M & RYR Protein,His,Avitag™&Tag Free

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    HLA-C*07:02:01 & B2M & RYR
  • Source
    Biotinylated Human HLA-C*07:02:01 & B2M & RYR ,His,Avitag™&Tag Free (HLR-H52W2) is expressed from human 293 cells (HEK293). It contains AA Cys 25 – Ile 308 | Ile 21 - Met 119 (Accession # P10321-1| RYRPGTVAL & P61769-1).
    Predicted N-terminus: Cys 25 | Arg
  • Molecular Characterization

    This protein .

    The protein has a calculated MW of 36.1 kDa | 13.8 kDa. The protein migrates as 42-45 kDa and 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human HLA-C*07:02:01 & B2M & RYR ,His,Avitag™&Tag Free  (Cat. No. HLR-H52W2) SDS-PAGE gel

Biotinylated Human HLA-C*07:02:01 & B2M & RYR ,His,Avitag™&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Biotinylated Human HLA-C*07:02:01 & B2M & RYR ,His,Avitag™&Tag Free (Cat. No. HLR-H52W2) immobilized on SA Chip can bind Human KIR2DL3, Fc Tag (Cat. No. KI3-H5258) with an affinity constant of 2.40 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Biotinylated Human HLA-C*07:02:01 & B2M & RYR ,His,Avitag™&Tag Free (Cat. No. HLR-H52W2) immobilized on SA Chip can bind Human KIR2DL2, Fc Tag (Cat. No. KI2-H5255) with an affinity constant of 1.01 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    HLA-A, B, and C are transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. The C receptor is a heterodimer consisting of a HLA-C mature gene product (heavy chain) and β2-microglobulin (light chain). The mature C chain is anchored in the membrane. HLA class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. HLA-C are expressed in nearly all cells, and present small peptides to the immune system which surveys for non-self peptides.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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