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Your Position: Home > CAR-T > FMC63 > FM3-PY54A2

PE-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) (Site-specific conjugation) DMF Filed (0.03% Proclin)

  • Source
    PE-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with FMC63.
  • Isotype
    IgG1/kappa
  • Specificity
    Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.
  • Application
    Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression). Please note that this product is NOT compatible to streptavidin detection system.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, 0.03% Proclin, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months after reconstitution;
    3. 2-8°C for 12 months after reconstitution.
Bioactivity-FACS
 FMC63 FACS

5e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Anti FMC63 scFv Antibody, Mouse IgG1 (Cat. No. FM3-PY54A2) and isotype control antibody respectively. PE signal was used to evaluate the binding activity (QC tested).

 FMC63 FACS

Binding activity of the PE-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 before and after adding 0.03% Proclin was evaluated in the above FACS analysis. The result shows that 0.03% Proclin (Cat. No. FM3-PY54A2) and Preservative free (Cat. No. FM3-HPY53) PE-Labeled Monoclonal Anti-FMC63 scFv Antibody have the same binding activity against anti-CD19 CAR-293 cells.

  • Background
    FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 2 business days

Price(USD) : $359.00

Price(USD) : $2178.00

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DMF (Drug Master File)

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