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Your Position: Home > > GDP-Fuc-vc-PAB-MMAE > ADC-P001

AGlink ADC Conjugation Kit (MMAE, DAR4)

For research use only.
IDComponentsSize
ADC-P001-C0120 x Reaction buffer30 μL
ADC-P001-C02Cofactor A12 μL
ADC-P001-C03Cofactor B12 μL
ADC-P001-C04Substrate A30 μL
ADC-P001-C05Substrate B24 μL
ADC-P001-C06Enzyme A150 μL
ADC-P001-C07Enzyme B60 μL
ADC-P001-1-C01Binding buffer15 mL
ADC-P001-1-C02Elution buffer1.5 mL
ADC-P001-1-C03Neutralization buffer0.5 mL
ADC-P001-1-C04250 mM Tris-HCl buffer (pH7.5)1.5 mL
ADC-P001-1-C0510×PBS buffer (pH7.2-7.4)6 mL
ADC-P001-1-C06Desalting column2 per
ADC-P001-1-C07Concentrator tube2 per
ADC-P001-1-C08ProteinA Resin200 μL
  • Background
    Antibody-drug conjugates (ADC) is a type of fast growing anticancer drug. Monoclonal antibody is conjugated to the cytotoxic payload via a chemical linker that directed toward a target antigen expressed on the cancer cell surface, reducing systemic exposure and therefore toxicity. AGlink ADC conjugation kit is designed to conjugate mAb with toxic payload (MMAE) though site-specific enzymatic reaction.
  • Application

    AGlink ADC Conjugation Kit is designed to conjugate mAb with toxic payload (MMAE) though site-specific enzymatic reaction. 1 reaction can conjugate 2mg antibody.

    It is for research use only.

  • Storage
    BoxA: The unopened kit is stable for 12 months from the date of manufacture if stored under temperature ≤ -20°C. The opened kit should be stored according to components table.

    BoxB: The unopened kit is stable for 12 months from the date of manufacture if stored under 4°C. The opened kit should be stored according to components table.

  • Assay Principles
    Almost all monoclonal antibodies are glycosylated at Asn-297 of Fc domain. While the glycans have different isoforms, the types which typically dominate are G0F, G1F&G2F (>90%). AGlink® ADC kit, which is based on the YTConju™ Platform of Glyco-therapy Biotechnology, employs the N-linked glycosylation site for site-specific toxin conjugation. Firstly, galactose is added to the terminal GlcNAc catalyzed by β-1,4-galactosyltransferase (GT) to form LacNAc. Then, the α-1,3-fucosyltransferase (FT) recognize LacNAc, and the toxin is transferred to the GlcNAc of LacNAc. So, the theoretical DAR value of the final conjugates is 4. The conjugation procedure, which is enzyme catalyzed, is performed as one-pot by combining glycan modification and toxin conjugation simultaneously. The conjugation procedure, which is easy to operate, is performed under physiological conditions, and organic solvents are not introduced. The conjugates prepared through this method usually have high homogeneity and the binding activity of the conjugates to related antigens is not changed.
Typical Data Please refer to DS document for The assay protocol.
 GDP-Fuc-vc-PAB-MMAE ELISA

ADC DAR value assessment by RP-HPLC
DAR (Drug Antibody Ratio) value of ADC product was analyzed by RP-HPLC (QC tested). Column: TSKgel Butyl-NPR column (4.6 mm × 35 mm, 2.5 μm; TOSOH) Mobile phase A: 20 mM sodium phosphate, 1.5 M ammonium sulfate (pH 6.9) Mobile phase B: 75% (v/v) 20 mM sodium phosphate, 25% (v/v) isopropanol (pH 6.9) Flow rate: 0.4 mL/min Column temperature: 30 °C Detection wavelength: 280 nm Loading Amount: 10 µg (5-10 µL)

 GDP-Fuc-vc-PAB-MMAE ELISA

DAR (Drug Antibody Ratio) value of ADC product was analyzed by RP-HPLC (QC tested). DAR=(1.39 x 0 + 2.23 x 1 + 0.98 x 2 + 3.00 x 3 + 92.41 x 4) / 100 = 3.84

  • Statement of Rights
    AGLink products are co-developed by ACROBiosystems Co., Ltd. and Glyco-therapy Biotechnology Co., Ltd. based on Glyco-therapy's conjugation platform (YTConju™) and are not authorized for commercial use. The sale and use of AGLink products doesn’t represent any forms of license or authorization of the attached technologies for developing any diagnostic, therapeutic or prophylactic products for commercial purposes
  • Note of application
    AGLink products are intended for research use only by the Buyer or its users and can not be used for any other purposes, including, but not limited to, use for any clinical diagnostic type use, in vitro or in vivo therapeutic use, prophylactic use, any type of consumption or application to human or animals.

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Price(USD) : $2000.00

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