This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™)
The protein has a calculated MW of 66 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human IL-1 RAcP / IL-1 R3 Protein,Fc,Avitag™ (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human IL-1 RAcP / IL-1 R3 Protein,Fc,Avitag™ (MALS verified) (Cat. No. ILP-H82F5) is more than 85% and the molecular weight of this protein is around 155-175kDa verified by SEC-MALS.
Immobilized Human Anti-IL1RAP Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human IL-1 RAcP / IL-1 R3 ,Fc,Avitag (Cat. No. ILP-H82F5) with a linear range of 0.5-16 ng/mL (QC tested).
Immobilized Biotinylated Human IL-1 RAcP / IL-1 R3 ,Fc,Avitag (Cat. No. ILP-H82F5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Anti-IL1RAP Human IgG1 with a linear range of 0.2-4 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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