Login Register
icon_bulk_orderBulk order Acrobiosystems for English
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > HIV Platform Pseudovirus > VSV-G Protein > PVGI-HLC027

VSV-G (Indiana) Fluc Pseudovirus

This product is still under development. Please contact us if you have interest in this product. We will accelerate the development process accordingly and reserve this product for you as request.
Abbreviations VSV-G (Indiana) Fluc Pseudovirus
Backbone HIV-1
Envelope protein VSV Glycoprotein (Indiana)
Reporter Firefly luciferase
Physical appearance Light red to light yellow transparent liquid
Storage -70°C
Transport Dry ice
Application Control of Neutralization assay
  • Application

    Pseudovirus Neutralization Assay The product is used to be a control of the neutralization assay of HIV-1 based SARS-CoV-2 pseudovirus, such as Cat. No. PSSO-HLC012, PPO-HLC014, PSSO-HLC015, PSSO-HLC016, PSSO-HLC017, etc. a. Mix 89% DMEM medium, 10% Fetal bovine serum and 1% Penicillin-Streptomycin to prepare complete DMEM medium. b. Thaw the pseudovirus at room temperature. c. Dilute your samples with complete DMEM medium in a 96-well white flat bottom plate to reach a volume of 50 ~ 140 μL per well, then add 10 ~ 50 μL diluted pseudovirus suspension per well to reach a final volume of 100 ~ 150 μL per well. Gently flap to mix well. Incubate the plate in a 5% (vol/vol) CO2, 37°C incubator for 60 min. d. Digest and resuspend HEK293/Human ACE2 Overexpression Stable Cells (ACROBiosystems, Cat. No. CHEK-ATP042) with complete DMEM medium. Adjust the cell density to 4 ~ 5 × 105 cells per milliliter with complete DMEM medium. Seed 100 μL the cell suspension per well into the 96-well plate. Gently flap to mix well. Incubate the plate in a 5% (vol/vol) CO2, 37°C incubator for 48 h. Notably, if a higher signal value is needed, an alternative parameter combination, 2 × 105 per milliliter (cell density) and 72 h (incubation time), can be tried. e. Prepare the detection reagent (britelite plus Reporter Gene Assay System (PerkinElmer, Cat. No. 6066761)) and balance it to room temperature. f. Take out the 96-well plate and discard 100 ~ 150 μL medium per well. Balance the plate to room temperature for 10 min. Add 100 μL detection reagent and mix well. Incubate for 2 min at room temperature. g. Read the luminescence values (RLU) of the wells with a luminescence meter (PerkinElmer, Cat. No. HH34000000).

  • Background

    Pseudovirus Neutralization Assay The product is used to be a control of the neutralization assay of HIV-1 based SARS-CoV-2 pseudovirus, such as Cat. No. PSSO-HLC012, PPO-HLC014, PSSO-HLC015, PSSO-HLC016, PSSO-HLC017, etc. a. Mix 89% DMEM medium, 10% Fetal bovine serum and 1% Penicillin-Streptomycin to prepare complete DMEM medium. b. Thaw the pseudovirus at room temperature. c. Dilute your samples with complete DMEM medium in a 96-well white flat bottom plate to reach a volume of 50 ~ 140 μL per well, then add 10 ~ 50 μL diluted pseudovirus suspension per well to reach a final volume of 100 ~ 150 μL per well. Gently flap to mix well. Incubate the plate in a 5% (vol/vol) CO2, 37°C incubator for 60 min. d. Digest and resuspend HEK293/Human ACE2 Overexpression Stable Cells (ACROBiosystems, Cat. No. CHEK-ATP042) with complete DMEM medium. Adjust the cell density to 4 ~ 5 × 105 cells per milliliter with complete DMEM medium. Seed 100 μL the cell suspension per well into the 96-well plate. Gently flap to mix well. Incubate the plate in a 5% (vol/vol) CO2, 37°C incubator for 48 h. Notably, if a higher signal value is needed, an alternative parameter combination, 2 × 105 per milliliter (cell density) and 72 h (incubation time), can be tried. e. Prepare the detection reagent (britelite plus Reporter Gene Assay System (PerkinElmer, Cat. No. 6066761)) and balance it to room temperature. f. Take out the 96-well plate and discard 100 ~ 150 μL medium per well. Balance the plate to room temperature for 10 min. Add 100 μL detection reagent and mix well. Incubate for 2 min at room temperature. g. Read the luminescence values (RLU) of the wells with a luminescence meter (PerkinElmer, Cat. No. HH34000000).

  • Basic advice

    Basic advice
    a. Though pseudovirus particles has no pathogenicity and cannot replicate, the assays should be carried out carefully in a Biosafety Level 2 or higher-level laboratory with a biosafety cabinet.
    b. Serum samples from animals or humans should be inactivated in a water bath at 56°C for 30 min before being tested.
    c. Please avoid freezing and thawing, which would influence the titer of the pseudovirus.
    d. The product is for Research Use Only.

    For more information and advice, please check the Data Sheet of this product.

    Optional Relative Products

Comments (0)

Price(USD) : $460.00

Promotion & Exhibitions



Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America: +1 800-810-0816 (Toll Free)
Asia & Pacific: +86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message