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Your Position: Home > Non SARS-Cov-2 Kits > IL-17A > EP-140

IL-17A : IL-17RA[Biotinylated] Inhibitor Screening ELISA Kit

For research use only.
IDComponentsSize
EP140-C01High-bind Plate1 plate
EP140-C02Human IL-17A10 μg
EP140-C03Biotinylated Human IL-17RA10 μg
EP140-C04Anti-IL-17A Neutralizing Antibody20 μg
EP140-C05Streptavidin-HRP10 μg
EP140-C06Coating Buffer12 mL
EP140-C0710xWashing Buffer 50 mL
EP140-C08Blocking Buffer50 mL
EP140-C09Substrate Solution12 mL
EP140-C10Stop Solution12 mL
  • Background
    Interleukin 17 receptor A (IL17RA) is also known as cluster of differentiation w217 (CDw217), is a pro-inflammatory cytokine secreted by activated T-lymphocytes, belong to ubiquitous type I membrane glycoprotein, and binds with low affinity to interleukin 17A (IL17A). IL 17R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested . IL 17RA associates with IL 17RC to form a signaling receptor complex for IL 17 and IL 17F . Ligand and IL 17RA ligation promotes T cell activation and the production of IL - 6, G-CSF, SCF, and multiple pro-inflammatory chemokines. Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5).
  • Application
    This kit is developed for screening for inhibitors of human IL-17A binding to human IL-17RA.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new IL-17A pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human IL-17RA to immobilized human IL-17A in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated IL-17RA protein, a human IL-17A protein, an anti-IL-17A neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

    Your experiment will include 4 simple steps:

    a) Coat the plate with human IL-17A.

    b) Add your molecule of interest to the tests.

    c) Add human IL-17 RA-Biotin to bind the coated IL-17 A.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the half maximal inhibitory concentration (IC50) of your compound to IL-17 RA: IL-17A binding will be determined by comparing OD readings among different experimental groups.

Typical Data
 IL-17A ELISA

INHIBITION OF IL-17A: IL-17RA[Biotinylated] BINDING BY ANTI-IL-17A NEUTRALIZING ANTIBODY
Serial dilutions of Anti-IL-17A Neutralizing antibody (Catalog # EP140-C04) (1:1 serial dilution, from 20 μg/mL to 0.01μg/mL) was added into IL-17A: Biotinylated IL-17RA binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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ETD of in-stock products: 2 business days

Price(USD) : $650.00

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