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Your Position: Home > TME Relevant Pathway > Laminin 521 E8 > LA5-H5261

Human Laminin 521 E8 Protein, Fc Tag (MALS verified)

  • Synonym
    Laminin 521 E8,LN-521-E8,Laminin-521 E8
  • Source
    Human Laminin 521 E8, Fc Tag(LA5-H5261) is expressed from human 293 cells (HEK293). It contains AA Glu 2655 – Ala 3327 (Laminin alpha5E8) & Leu 1573 - Gln 1798 (Laminin beta2E8) & Asp 1528 – Pro 1609 (Laminin gamma1E8) (Accession # O15230-1 (Laminin alpha5E8) & P55268-1 (Laminin beta2E8) & P11047-1 (Laminin gamma1E8)).
    Predicted N-terminus: Glu 2655 (Laminin alpha5E8) & Pro (Laminin beta2E8) & Pro (Laminin gamma1E8)
  • Molecular Characterization

    This protein carries a human IgG1 Fc tag at the N-terminus

    The protein has a calculated MW of 76.7 kDa | 51.3 kDa | 35.6 kDa. The protein migrates as 92-97 kDa and 100-116 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Laminin 521 E8 SDS-PAGE

Human Laminin 521 E8, Fc Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Laminin 521 E8 MALS images

The purity of Human Laminin 521 E8, Fc Tag (Cat. No. LA5-H5261) is more than 85% and the molecular weight of this protein is around 160-185kDa verified by SEC-MALS.

Bioactivity-ELISA
 Laminin 521 E8 ELISA

Immobilized Human Laminin 521 E8, Fc Tag (Cat. No. LA5-H5261) at 5 μg/mL (100 μL/well) can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with a linear range of 0.01-0.156 μg/mL (QC tested).

 Laminin 521 E8 ELISA

Immobilized Human Laminin 521 E8, Fc Tag (Cat. No. LA5-H5261) at 5 μg/mL (100 μL/well) can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) with a linear range of 0.02-0.313 μg/mL (Routinely tested).

Bioactivity-BLI
 Laminin 521 E8 BLI

Loaded Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) on HIS1K Biosensor, can bind Human Laminin 521 E8, Fc Tag (Cat. No. LA5-H5261) with an affinity constant of 4.31 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Laminin 521 were expressed in Descemets membrane and corneal endothelium. This laminin isoform significantly enhanced the in vitro adhesion and proliferation, and differentiation of HCECs. Laminin 511-E8 consists of the C-terminal end of the alpha 5, beta 2, and gamma 1 chains.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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