This protein carries a human IgG1 Fc tag at the N-terminus
The protein has a calculated MW of 76.7 kDa | 51.3 kDa | 35.6 kDa. The protein migrates as 92-97 kDa and 100-116 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Laminin 521 E8, Fc Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human Laminin 521 E8, Fc Tag (Cat. No. LA5-H5261) is more than 85% and the molecular weight of this protein is around 160-185kDa verified by SEC-MALS.
Immobilized Human Laminin 521 E8, Fc Tag (Cat. No. LA5-H5261) at 5 μg/mL (100 μL/well) can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with a linear range of 0.01-0.156 μg/mL (QC tested).
Immobilized Human Laminin 521 E8, Fc Tag (Cat. No. LA5-H5261) at 5 μg/mL (100 μL/well) can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Loaded Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) on HIS1K Biosensor, can bind Human Laminin 521 E8, Fc Tag (Cat. No. LA5-H5261) with an affinity constant of 4.31 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $400.00
Price(USD) : $2600.00
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