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This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 102.4 kDa. The protein migrates as 130 kDa±5 kDa under reducing (R) condition, and ＞250 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human VCAM-1, Fc,Avitag, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Human VCAM-1, Fc,Avitag, premium grade (Cat. No. VC1-H82F6) is more than 90% and the molecular weight of this protein is around 210-250 kDa verified by SEC-MALS.
Immobilized Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human VCAM-1, Fc,Avitag, premium grade (Cat. No. VC1-H82F6) with a linear range of 0.1-13 ng/mL (QC tested).
Immobilized Biotinylated Human VCAM-1, Fc,Avitag, premium grade (Cat. No. VC1-H82F6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W1) with a linear range of 0.02-0.313 μg/mL (Routinely tested).
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