|Standard Curve Range||3.90 pg/mL-500 pg/mL|
|Assay Time||2 hr|
|Suitable Sample Type||For the quantitative determination of human IL-12p70 in Plasma and Serum.|
|Sample volume||50 μL|
|CEA009-C01||Pre-coated Anti-IL-12 Ab Microplate||1 plate|
|CEA009-C02||IL-12p70 Calibrator||20 μg×2|
|CEA009-C03||Biotin-Anti-IL-12 Ab Con. Solution||100 μL|
|CEA009-C04||Biotin-Ab Dilution Buffer||8 mL|
|CEA009-C05||IL-12p70 SA-HRP Con. Solution||0.5 mL|
|CEA009-C06||SA-HRP Dilution Buffer||15 mL|
|CEA009-C07||20× Washing Buffer||50 mL|
|CEA009-C08||Sample Dilution Buffer||15 mL×2|
|CEA009-C09||Substrate Solution||12 mL|
|CEA009-C10||Stop Solution||6 mL|
ClinMax product are manufactured in a dedicated GMP facility and are compliant with our ISO 13485 quality management system.
The verification scheme was designed in accordance with the 9012 "Guidelines for the Verification of Quantitative Analysis Methods of Biological Samples" in the Chinese Pharmacopoeia 2020 Edition and the ICH M10 Guideline. Sensitivity, specificity, precision, recovery, and linearity were verified for multi-batch products and multiple concentration samples. See manual for more information on validation in DS.
Results are obtained by Log-Log Linear regression equation are used to draw the standard curve and calculate the sample concentration. The verification results indicate that this kit can be used for the quantitative determination of natural and recombinant human Interleukin 12 concentrations.
It has been calibrated against a highly purified human IL-12p70 and is evaluated with standard from NIBSC/WHO. Reference Reagent Interleukin-12 (Human, rDNA derived) NIBSC code: 95/544.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CEA-C009 is less than 1.0 pg/mL.
Three human serum samples with high concentrations of IL-12p70 were diluted 1:2, 1:4, 1:8, 1:16, 1:32,1:64 with Dilution Buffer to produce samples with values within the dynamic range and then assayed. On average, 106.25% of IL-12p70 was detected from serum samples.
Ten replicates of each of five samples containing different IL-12p70 concentrations were tested in one assay , Intra-Assay Precision CV＜10%.
Five samples containing different concentrations of IL-12p70 were tested in three independent assays , Inter-Assay Precision CV<15%.
Recombinant IL-12p70 was spiked into 5 human serum samples, and then analyzed. On average, 98.95% of IL-12p70 was recovered from serum samples.
Recombinant IL-12p70 was spiked into cell supernatant sample, and then analyzed. On average, 97.17% of IL-12p70 was recovered from serum samples.
Bilirubin (simulated jaundice) concentration should be less than 20mg/dL, hemoglobin (simulated hemolysis) concentration should not be higher than 3500mg/dL, triglyceride (simulated lipemia) concentration may not be higher than 2.0g/L, Heparin concentration should be less than 40U/mL, EDTA concentration should be less than 4mg/mL, and Sodium citrate concentration may not be higher than 40mg/mL, it does not affect the detection result.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $549.00
ComboX is a new brand of ACROBiosystems. ComboX products include our Streptavidin series products, coated plates, isotype controls for antibody and fusion protein drug research, and general-purpose antibodies.
ACROBiosystems developed a series of GMP grade cytokines under the GMP grade quality management system. Those products are all suitable for T/NK cell generation, activation, and proliferation in cell therapy research.
Emerging VoCs, Omicron, Delta, Beta, Alpha mutants and so on, including RBD, S trimer, S1, NTD, NP, etc. These mutants are of high purity and bioactivity and can be used to evaluate the efficacy of the antibodies and vaccination.
50+ targets designed for CAR detection, including PE/FITC/biotin labeled proteins. The key reagents for CD19 and BCMA were FDA DMF filed which can support your IND, NDA and BLA process.
GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
Full length multi-pass TPs with stabilized structure and high bioactivity for immunization, antibody screening, cell based assay and CAR detection, including hot CD20, Claudin 18.2， CD133, GPRC5D，CCR8, CCR5, etc.
CD3 proteins and a collection of for bispecific antibody development which are of high specificity and bioactivities and suitable for immunization, antibody screening.
A series of immune checkpoints including classic co-inhibitory and co-stimulatory receptors. The comprehensive catalog contains 100+ targets with various species and tags, and the high-quality proteins are in good batch-to-batch consistency.
To meet the needs of ADCs development, ACROBiosystems can provide: A variety of high-quality target proteins; MMPs/Cathepsin/uPA for cleavable linker; Anti-payload antibodies & anti-idiotypic antibodies for immunogenicity and PK analysis; SPR/BLI analytical and ADA development service.
Comprehensive collection of Fc receptor proteins, including their common variants, which can help expedite your antibody development.
Comprehensive cytokine targets including interleukins, growth factors, chemokines, TNFs, etc. are expressed by HEK293 to ensure their natural structure. Their high purity is verified by SDS-PAGE/HPLC/SEC-MALS and high bioactivity is verified by ELISA/SPR/BLI.
Aneuro is ACROBiosystems brand focused on neuroscience research, providing high-quality important proteins including therapeutic and diagnostic research proteins, PFFs and recombinant neuro factors, etc. to advance neuroscience research.
This web search service is supported by Google Inc.