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Your Position: Home > Non SARS-Cov-2 Kits > IL-12 (P70) > CEA-C009

ClinMax™ Human IL-12p70 ELISA Kit

For research use only.
Product details
Assay Type Sandwich-ELISA
Analyte IL-12p70
Format 96T
Reactivity Human
Regulatory Status RUO
Sensitivity <1.0 pg/mL
Standard Curve Range 3.90 pg/mL-500 pg/mL
Assay Time 2 hr
Suitable Sample Type For the quantitative determination of human IL-12p70 in Plasma and Serum.
Sample volume 50 μL
Materials provided
IDComponentsSize
CEA009-C01Pre-coated Anti-IL-12 Ab Microplate1 plate
CEA009-C02IL-12p70 Calibrator20 μg×2
CEA009-C03Biotin-Anti-IL-12 Ab Con. Solution100 μL
CEA009-C04Biotin-Ab Dilution Buffer8 mL
CEA009-C05IL-12p70 SA-HRP Con. Solution0.5 mL
CEA009-C06SA-HRP Dilution Buffer15 mL
CEA009-C0720× Washing Buffer50 mL
CEA009-C08Sample Dilution Buffer15 mL×2
CEA009-C09Substrate Solution12 mL
CEA009-C10Stop Solution6 mL
  • Background
    ClinMax™ Human IL-12p70 Quantitative ELISA Kit is a one-step quick immunoassay designed to quantitate human IL-12p70 that is present in complex biological matrices, such as human serum, plasma, buffered solution. This assay kit is a standard sandwich-ELISA format.

    ClinMax product are manufactured in a dedicated GMP facility and are compliant with our ISO 13485 quality management system.

    The verification scheme was designed in accordance with the 9012 "Guidelines for the Verification of Quantitative Analysis Methods of Biological Samples" in the Chinese Pharmacopoeia 2020 Edition and the ICH M10 Guideline. Sensitivity, specificity, precision, recovery, and linearity were verified for multi-batch products and multiple concentration samples. See manual for more information on validation in DS.

  • Application
    Results are obtained by Log-Log Linear regression equation are used to draw the standard curve and calculate the sample concentration. The verification results indicate that this kit can be used for the quantitative determination of natural and recombinant human Interleukin 12 concentrations. It has been calibrated against a highly purified human IL-12p70 and is evaluated with standard from NIBSC/WHO. Reference Reagent Interleukin-12 (Human, rDNA derived) NIBSC code: 95/544.

    It is for research use only.

  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
     IL-12 (P70) Assay Principles
Typical Data
 IL-12 (P70) ELISA

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CEA-C009 is less than 1.0 pg/mL.

Validation
Dilution Linearity

Three human serum samples with high concentrations of IL-12p70 were diluted 1:2, 1:4, 1:8, 1:16, 1:32,1:64 with Dilution Buffer to produce samples with values within the dynamic range and then assayed. On average, 106.25% of IL-12p70 was detected from serum samples.

 IL-12 (P70) DILUTION LINEARITY
Intra-Assay Statistics

Ten replicates of each of five samples containing different IL-12p70 concentrations were tested in one assay , Intra-Assay Precision CV<10%.

 IL-12 (P70) INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Five samples containing different concentrations of IL-12p70 were tested in three independent assays , Inter-Assay Precision CV<15%.

 IL-12 (P70) INTER-ASSAY STATISTICS
Recovery

Recombinant IL-12p70 was spiked into 5 human serum samples, and then analyzed. On average, 98.95% of IL-12p70 was recovered from serum samples.

 IL-12 (P70) RECOVERY

Recombinant IL-12p70 was spiked into cell supernatant sample, and then analyzed. On average, 97.17% of IL-12p70 was recovered from serum samples.

 IL-12 (P70) RECOVERY
Interference effect

Bilirubin (simulated jaundice) concentration should be less than 20mg/dL, hemoglobin (simulated hemolysis) concentration should not be higher than 3500mg/dL, triglyceride (simulated lipemia) concentration may not be higher than 2.0g/L, Heparin concentration should be less than 40U/mL, EDTA concentration should be less than 4mg/mL, and Sodium citrate concentration may not be higher than 40mg/mL, it does not affect the detection result.

 IL-12 (P70) INTERFERENCE EFFECT
  • Background: IL-12 (P70)
    Interleukin 12, IL-12(P70), IL-12p70
    Biologically active IL-12 is a heterodimeric cytokine composed of p35 and p40 subunits (also known as IL-12p70). IL-12 is mainly produced by activated monocytes, macrophages, and DCs. IL-12, through STAT4 activation, is essential for the differentiation and maintenance of Th1 effector cells, which then produce high levels of IFN-γ, TNF-α, and IL-2. These cytokines, in turn, promote T-cell responses and macrophage activation that contribute to inflammatory responses. IL-12 is also important for enhancing the cytotoxicity mediated by NK cells. IL-12 has been shown to play a critical role in the pathogenesis of immune-related diseases, particularly autoimmune and inflammatory conditions such as RA, atherosclerosis, and MS.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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