>Immune Checkpoint Proteins
>Human PD-L1 / B7-H1 Protein, Fc Tag (HPLC-verified)
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 51.4 kDa. The protein migrates as 60-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human PD-L1, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) was greater than 90% as determined by SEC-HPLC.
Flow Cytometry assay shows that recombinant Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 10 μg/mL (Routinely tested).
FACS analysis shows that the binding of Human PD-L1, Fc Tag (HPLC-verified) to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing Anti-PD-L1 antibody. The concentration of PD-L1 used is 10 μg/mL. The IC50 is 12.92 μg/mL (Routinely tested).
Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) with an EC50 of 0.029 μg/mL (Routinely tested).
Authors: Boohaker RJ, et al.
Journal: Cancer Lett 2018
Authors: Yam Alice, et al.
Journal: US20180142022A1 2018
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